Hub-organized parallel circuits of central circadian pacemaker neurons for visual photoentrainment in Drosophila
| Autor: | Min Tang, Dong Gen Luo, Tian Yang, Taishi Yoshii, Li Hui Cao, Na Xiao, Bowen Deng, Meng Tong Li |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
genetic structures Science Circadian clock General Physics and Astronomy Biology Series and parallel circuits Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Biological Clocks Neuropil medicine Animals Drosophila Proteins Visual Pathways Circadian rhythm lcsh:Science Medulla Circadian pacemaker Neurons Multidisciplinary General Chemistry Circadian Rhythm 030104 developmental biology medicine.anatomical_structure lcsh:Q Drosophila Master clock sense organs Neuron Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | Circadian rhythms are orchestrated by a master clock that emerges from a network of circadian pacemaker neurons. The master clock is synchronized to external light/dark cycles through photoentrainment, but the circuit mechanisms underlying visual photoentrainment remain largely unknown. Here, we report that Drosophila has eye-mediated photoentrainment via a parallel pacemaker neuron organization. Patch-clamp recordings of central circadian pacemaker neurons reveal that light excites most of them independently of one another. We also show that light-responding pacemaker neurons send their dendrites to a neuropil called accessary medulla (aMe), where they make monosynaptic connections with Hofbauer–Buchner eyelet photoreceptors and interneurons that transmit compound-eye signals. Laser ablation of aMe and eye removal both abolish light responses of circadian pacemaker neurons, revealing aMe as a hub to channel eye inputs to central circadian clock. Taken together, we demonstrate that the central clock receives eye inputs via hub-organized parallel circuits in Drosophila. The central circadian clock in Drosophila is made up of ~ 150 anatomically distributed neurons; the circuits underlying photoentrainment is unclear. This study describes ex vivo patch-clamp recording of the eye-mediated light response of all known circadian clock neurons, and shows that they are organized in parallel circuits centered around a hub. |
| Databáze: | OpenAIRE |
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