Laser-assisted cell removing (LACR) technology contributes to the purification process of the undifferentiated cell fraction during pluripotent stem cell culture
| Autor: | Hirofumi Suemori, Toshiyuki Takehara, Koichi Suzuki, Koichi Matsuda, Kenji Shinohara, Tatsufumi Mori, Yuji Miyashita, Yasumichi Mieno, Kanji Fukuda, Takeshi Teramura |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pluripotent Stem Cells Infrared Rays Cellular differentiation Cell Induced Pluripotent Stem Cells Biophysics Cell Culture Techniques Regenerative Medicine Biochemistry Regenerative medicine Cell Line 03 medical and health sciences Mice medicine Animals Humans Undifferentiated cell Induced pluripotent stem cell Molecular Biology Process (anatomy) Cell Death Chemistry Lasers Cell Differentiation Cell Biology Laser assisted Coculture Techniques Cell biology Trophoblasts 030104 developmental biology medicine.anatomical_structure Stem cell |
| Zdroj: | Biochemical and biophysical research communications. 503(4) |
| ISSN: | 1090-2104 |
| Popis: | Purification of undifferentiated cells by removing differentiated parts is an essential step in pluripotent stem cell culture. This process has been traditionally performed manually using a fine glass capillary or plastic tip under a microscope, or by culturing in a selective medium supplemented with anti-differentiation inhibitors. However, there are several inevitable problems associated with these methods, such as contamination or biological side-effects. Here, we developed a laser-assisted cell removing (LACR) technology that enables precise, fast, and contact-less cell removal. Using LACR combined with computational image recognition/identification-discriminating technology, we achieved automatic cell purification (A-LACR). Practicability of A-LACR was evaluated by two demonstrations: selective removal of trophoblast stem (TS) cells from human iPS and TS cell co-cultures, and purification of undifferentiated iPS cells by targeting differentiated cells that spontaneously developed. Our results suggested that LACR technology is a novel approach for stem cell processing in regenerative medicine. |
| Databáze: | OpenAIRE |
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