Layer-specific potentiation of network GABAergic inhibition in the CA1 area of the hippocampus
| Autor: | Filippo Drago, Michelangelo Colavita, Geoffrey Terral, Federico Massa, Giovanni Marsicano, Clement E. Lemercier |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Patch-Clamp Techniques Glycine In Vitro Techniques Biology Hippocampal formation Receptors Metabotropic Glutamate Inhibitory postsynaptic potential Hippocampus Article Mice 03 medical and health sciences Glutamatergic 0302 clinical medicine Postsynaptic potential Animals Inositol 1 4 5-Trisphosphate Receptors Multidisciplinary Long-term potentiation Resorcinols Anatomy Synaptic Potentials Receptors GABA-A Mice Inbred C57BL 030104 developmental biology Metabotropic glutamate receptor Synaptic plasticity GABAergic Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep28454 |
| Popis: | One of the most important functions of GABAergic inhibition in cortical regions is the tight control of spatiotemporal activity of principal neuronal ensembles. However, electrophysiological recordings do not provide sufficient spatial information to determine the spatiotemporal properties of inhibitory plasticity. Using Voltage Sensitive Dye Imaging (VSDI) in mouse hippocampal slices, we demonstrate that GABAA-mediated field inhibitory postsynaptic potentials undergo layer-specific potentiation upon activation of metabotropic glutamate receptors (mGlu). VSDI recordings allowed detection of pharmacologically isolated GABAA-dependent hyperpolarization signals. Bath-application of the selective group-I mGlu receptor agonist, (S)-3,5-Dihydroxyphenylglycine (DHPG), induces an enhancement of the GABAergic VSDI-recorded signal, which is more or less pronounced in different hippocampal layers. This potentiation is mediated by mGlu5 and downstream activation of IP3 receptors. Our results depict network GABAergic activity in the hippocampal CA1 region and its sub-layers, showing also a novel form of inhibitory synaptic plasticity tightly coupled to glutamatergic activity. |
| Databáze: | OpenAIRE |
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