Characterization of retinal biomechanical properties using Brillouin microscopy
Autor: | Yogeshwari S. Ambekar, Benjamin M. Hall, Giuliano Scarcelli, Elda M. Rueda, Kirill V. Larin, Manmohan Singh, Ross A. Poché |
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Rok vydání: | 2020 |
Předmět: |
Retinal Ganglion Cells
retina Materials science N-Methylaspartate Letter retinal damage genetic structures Biomedical Engineering Brillouin microscopy 01 natural sciences tissue biomechanics 010309 optics Biomaterials chemistry.chemical_compound Mice Optical coherence tomography 0103 physical sciences Microscopy medicine Animals Vision Ocular Retina Brillouin Spectroscopy medicine.diagnostic_test Stiffness Retinal JBO Letters Atomic and Molecular Physics and Optics eye diseases Electronic Optical and Magnetic Materials Brillouin zone medicine.anatomical_structure Retinal ganglion cell chemistry N-methyl-D-aspartate sense organs medicine.symptom Biomedical engineering |
Zdroj: | Journal of Biomedical Optics |
ISSN: | 1560-2281 |
Popis: | Significance: The retina is critical for vision, and several diseases may alter its biomechanical properties. However, assessing the biomechanical properties of the retina nondestructively is a challenge due to its fragile nature and location within the eye globe. Advancements in Brillouin spectroscopy have provided the means for nondestructive investigations of retina biomechanical properties. Aim: We assessed the biomechanical properties of mouse retinas using Brillouin microscopy noninvasively and showed the potential of Brillouin microscopy to differentiate the type and layers of retinas based on stiffness. Approach: We used Brillouin microscopy to quantify stiffness of fresh and paraformaldehyde (PFA)-fixed retinas. As further proof-of-concept, we demonstrated a change in the stiffness of a retina with N-methyl-D-aspartate (NMDA)-induced damage, compared to an undamaged sample. Results: We found that the retina layers with higher cell body density had higher Brillouin modulus compared to less cell-dense layers. We have also demonstrated that PFA-fixed retina samples were stiffer compared with fresh samples. Further, NMDA-induced neurotoxicity leads to retinal ganglion cell (RGC) death and reactive gliosis, increasing the stiffness of the RGC layer. Conclusion: Brillouin microscopy can be used to characterize the stiffness distribution of the layers of the retina and can be used to differentiate tissue at different conditions based on biomechanical properties. |
Databáze: | OpenAIRE |
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