Maternal overnutrition before and during pregnancy induces DNA damage in male offspring: A rabbit model
| Autor: | Pablo Pánico, Andrea Díaz-Villaseñor, Patricia Ostrosky-Wegman, Rodrigo Montúfar-Chaveznava, Monserrat Sordo, Erika Navarrete-Monroy, Ana María Salazar, Ivette Caldelas |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Offspring Health Toxicology and Mutagenesis Physiology Diet High-Fat medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound Overnutrition 0302 clinical medicine Pregnancy Genetics medicine Animals Weaning Sex Characteristics business.industry Maternal Nutritional Physiological Phenomena medicine.disease Malondialdehyde Pregnancy Complications Disease Models Animal Oxidative Stress 030104 developmental biology chemistry In utero Prenatal Exposure Delayed Effects 030220 oncology & carcinogenesis Gestation Female Rabbits business Oxidative stress DNA Damage |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 865:503324 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/j.mrgentox.2021.503324 |
| Popis: | Using a rabbit model, we investigated whether maternal intake of a high-fat and high-carbohydrate diet (HFCD) before and during pregnancy induces an increase in micronuclei frequency and oxidative stress in offspring during adulthood. Female rabbits received a standard diet (SD) or HFCD for two months before mating and during gestation. The offspring from both groups were nursed by foster mothers fed SD until postnatal day 35. After weaning, all the animals received SD until postnatal day 440. At postnatal day 370, the frequency of micronuclei in peripheral blood reticulocytes (MN-RETs) increased in the male offspring from HFCD-fed mothers compared with the male offspring from SD-fed mothers. Additionally, fasting serum glucose increased in the offspring from HFCD-fed mothers compared with the offspring from SD-fed mothers. At postnatal day 440, the offspring rabbits were challenged with HFCD or continued with SD for 30 days. There was an increase in MN-RET frequency in the male rabbits from HFCD-fed mothers, independent of the type of challenging diet consumed during adulthood. The challenge induced changes in serum cholesterol, LDL and HDL that were influenced by the maternal diet and offspring sex. We measured malondialdehyde in the liver of rabbits as an oxidative stress marker after diet challenge. Oxidative stress in the liver only increased in the female offspring from HFCD-fed mothers who were also challenged with this same diet. The data indicate that maternal overnutrition before and during pregnancy is able to promote different effects depending on the sex of the animals, with chromosomal instability in male offspring and oxidative stress and hypercholesterolemia in female offspring. Our data might be important in the understanding of chronic diseases that develop in adulthood due to in utero exposure to maternal diet. |
| Databáze: | OpenAIRE |
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