Insecticide resistance genes affect Culex quinquefasciatus vector competence for West Nile virus

Autor: Mawlouth Diallo, Mylène Weill, Laurence Mousson, Haoues Alout, Célestine M. Atyame, Marie Vazeille, Anna-Bella Failloux
Přispěvatelé: Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris], Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), This study was supported by the AXA research fund and the Pasteur-Cantarini postdoctoral fellowships. H.A. is supported by Marie Skołodowska-Curie under the grant agreement number 749897., We are very grateful to Sandra Unal, Patrick Makoundou and Jocelyne Alexandre for technical assistance., Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Arbovirus et Insectes Vecteurs, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Pesticide resistance
West Nile virus
viruses
030231 tropical medicine
Genes
Insect
Mosquito Vectors
medicine.disease_cause
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
parasitic diseases
medicine
Animals
genetics
Gene
030304 developmental biology
General Environmental Science
0303 health sciences
vector competence
General Immunology and Microbiology
biology
Ecology
virus diseases
Culex quinquefasciatus
General Medicine
Feeding Behavior
Subject Category: Ecology Subject Areas: ecology
insecticide resistance
Pesticide
biology.organism_classification
Virology
3. Good health
Culex
Insecticide resistance
microbiology Keywords: insecticide resistance
arboviruses
Vector (epidemiology)
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Female
General Agricultural and Biological Sciences
Disease transmission
West Nile Fever
Zdroj: Proceedings of the Royal Society B: Biological Sciences
Proceedings of the Royal Society B: Biological Sciences, Royal Society, The, 2019, 286 (1894), pp.20182273. ⟨10.1098/rspb.2018.2273⟩
Proceedings of the Royal Society B: Biological Sciences, 2019, 286 (1894), pp.20182273. ⟨10.1098/rspb.2018.2273⟩
ISSN: 0962-8452
1471-2954
Popis: Insecticide resistance has been reported to impact the interactions between mosquitoes and the pathogens they transmit. However, the effect on vector competence for arboviruses still remained to be investigated. We examined the influence of two insecticide resistance mechanisms on vector competence of the mosquito Culex quinquefasciatus for two arboviruses, Rift Valley Fever virus (RVFV) and West Nile virus (WNV). Three Cx. quinquefasciatus lines sharing a common genetic background were used: two insecticide-resistant lines, one homozygous for amplification of the Ester 2 locus (SA2), the other homozygous for the acetylcholinesterase ace-1 G119S mutation (SR) and the insecticide-susceptible reference line Slab. Statistical analyses revealed no significant effect of insecticide-resistant mechanisms on vector competence for RVFV. However, both insecticide resistance mechanisms significantly influenced the outcome of WNV infections by increasing the dissemination of WNV in the mosquito body, therefore leading to an increase in transmission efficiency by resistant mosquitoes. These results showed that insecticide resistance mechanisms enhanced vector competence for WNV and may have a significant impact on transmission dynamics of arboviruses. Our findings highlight the importance of understanding the impacts of insecticide resistance on the vectorial capacity parameters to assess the overall consequence on transmission.
Databáze: OpenAIRE
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