Proteomic Profiling of Diffuse Large B-Cell Lymphomas
| Autor: | Ann Kaufeldt, Svetlana Bajalica-Lagercrantz, Serhiy Souchelnytskyi, Catharina Larsson, Nazariy Souchelnytskyi, Anna Kwiecinska, Anna Porwit |
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| Rok vydání: | 2017 |
| Předmět: |
BiP/Grp78
0301 basic medicine Adult Male Proteomics Germinal center type Hsp90 Biology Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine immune system diseases hemic and lymphatic diseases medicine Biomarkers Tumor De novo diffuse large B-cell lymphomas Humans Cyclin B2 Non-germinal center type Molecular Biology Endoplasmic Reticulum Chaperone BiP B cell Aged Gel electrophoresis Aged 80 and over proteomics cancer Tissue microarray Proteomic Profiling Gene Expression Profiling Diffuse large B-cell lymphoma Cell Biology General Medicine Middle Aged medicine.disease Molecular biology Lymphoma Transformed lymphoma 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Child Preschool Proteome Female Lymphoma Large B-Cell Diffuse |
| Zdroj: | Pathobiology : journal of immunopathology, molecular and cellular biology. 85(4) |
| ISSN: | 1423-0291 |
| Popis: | Objective: The aim of this study was to identify differences in proteome profiles of diffuse large B-cell lymphoma (DLBCL) of nongerminal center (non-GC) versus GC type in the search for new markers and drug targets. Methods: Six DLBCL, with 3 repeats for each, were used for the initial study by proteomics: 3 non-GC and 3 GC DLBCL cases. For immunohistochemistry, tissue microarrays were made from 31 DLBCL samples: 16 non-GC de novo lymphomas and 15 GC cases (11 transformed from follicular lymphomas and 4 de novo GC lymphomas). Proteome profiling was performed by two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry. Results: Ninety-one proteins were found differentially expressed in non-GC compared to GC type. The Cytoscape tool was used for systemic analysis of proteomics data, revealing 19 subnetworks representing functions affected in non-GC versus GC types of DLBCL. Conclusion: A validation study of 3 selected proteins (BiP/Grp78, Hsp90, and cyclin B2) showed the enhanced expression in non-GC DLBCL, supporting the proteomics data. |
| Databáze: | OpenAIRE |
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