The Wnt pathway regulator DKK1 is preferentially expressed in hormone-resistant breast tumours and in some common cancer types

Autor: Jocelyne Martin, Jessica Godin-Ethier, Simon Turcotte, Sandy Pelletier, M-A Forget, Réal Lapointe, Diane Beauseigle, Simon Tanguay
Jazyk: angličtina
Rok vydání: 2007
Předmět:
CA15-3
musculoskeletal diseases
Male
Cancer Research
Pathology
medicine.medical_specialty
Lung Neoplasms
Antineoplastic Agents
Hormonal
Placenta
Breast Neoplasms
Sensitivity and Specificity
03 medical and health sciences
0302 clinical medicine
Breast cancer
breast cancer
Prostate
Cell Line
Tumor
medicine
Humans
RNA
Messenger
Lung cancer
Melanoma
030304 developmental biology
0303 health sciences
business.industry
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Wnt signaling pathway
Cancer
kidney cancer
Prostatic Neoplasms
Genetics and Genomics
medicine.disease
Kidney Neoplasms
3. Good health
Gene Expression Regulation
Neoplastic
lung cancer
medicine.anatomical_structure
Oncology
Drug Resistance
Neoplasm
030220 oncology & carcinogenesis
Cancer research
Dickkopf-1 (DKK1)
Intercellular Signaling Peptides and Proteins
prognostic and diagnostic marker
Female
business
Kidney cancer
Zdroj: British Journal of Cancer
ISSN: 1532-1827
0007-0920
Popis: In addition to new tumour antigens, new prognostic and diagnostic markers are needed for common cancers. In this study, we report the expression of Dickkopf-1 (DKK1) in multiple common cancers. This constitutes a comprehensive analysis of the DKK1 expression profile. Dickkopf-1 expression was evaluated by classical and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbant assay for protein determination, in cancer lines and clinical specimens of several cancer origins. For breast cancer, expression was correlated with clinicopathological parameters. Dickkopf-1 expression was confirmed in several cancer cell lines derived from breast and other common cancers. Dickkopf-1 protein secretion was documented in breast, prostate and lung cancer lines, but was negligible in melanoma. Analysis of DKK1 expression in human cancer specimens revealed DKK1 expression in breast (21 out of 73), lung (11 out of 23) and kidney cancers (six out of 20). Interestingly, DKK1 was preferentially expressed in oestrogen and progesterone receptor-negative tumours (ER(-)/PR(-); P=0.005) and in tumours from women with a family history of breast cancer (P=0.024). Importantly, DKK1 protein production was confirmed in multiple breast cancer specimens that were positive by RT-PCR. This work establishes DKK1 as a potential prognostic and diagnostic marker for cohorts of breast cancer patients with poor prognosis. Dickkopf-1 may also become a relevant candidate target for immunotherapy of different cancers.
Databáze: OpenAIRE
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