The centriolar satellite protein SSX2IP promotes centrosome maturation
| Autor: | Hideki Yokoyama, Sabine Merker, Justus Tegha-Dunghu, Stefanie Draeger, Dmytro Mayilo, Burkhard Hoeckendorf, Thomas Ruppert, Holger Lorenz, Joachim Wittbrodt, Maren Klinger, Stephanie Freiss, Ivana Cado, Daigo Inoue, Oliver J. Gruss, Herbert Steinbeisser, Kerstin Hupfeld, Felix Bärenz, Sahra Pilz |
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| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Blastomeres Embryo Nonmammalian Centriole Oryzias Mitosis Centrosome cycle Spindle Apparatus Xenopus Proteins Biology Time-Lapse Imaging Article Chromosomes Spindle pole body Xenopus laevis Tubulin Chromosome Segregation Animals Research Articles Centrioles Microtubule nucleation Pericentriolar material Centrosome Cell Biology Neoplasm Proteins Spindle apparatus Cell biology Repressor Proteins Gene Knockdown Techniques Oocytes Centriolar satellite |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | SSX2IP promotes centrosome maturation and maintenance at the onset of vertebrate development, preserving centrosome integrity and mitosis during rapid cleavage divisions and in somatic cells. Meiotic maturation in vertebrate oocytes is an excellent model system for microtubule reorganization during M-phase spindle assembly. Here, we surveyed changes in the pattern of microtubule-interacting proteins upon Xenopus laevis oocyte maturation by quantitative proteomics. We identified the synovial sarcoma X breakpoint protein (SSX2IP) as a novel spindle protein. Using X. laevis egg extracts, we show that SSX2IP accumulated at spindle poles in a Dynein-dependent manner and interacted with the γ-tubulin ring complex (γ-TuRC) and the centriolar satellite protein PCM-1. Immunodepletion of SSX2IP impeded γ-TuRC loading onto centrosomes. This led to reduced microtubule nucleation and spindle assembly failure. In rapidly dividing blastomeres of medaka (Oryzias latipes) and in somatic cells, SSX2IP knockdown caused fragmentation of pericentriolar material and chromosome segregation errors. We characterize SSX2IP as a novel centrosome maturation and maintenance factor that is expressed at the onset of vertebrate development. It preserves centrosome integrity and faithful mitosis during the rapid cleavage division of blastomeres and in somatic cells. |
| Databáze: | OpenAIRE |
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