Transcriptome analysis of IFM-specific actin and myosin nulls in Drosophila melanogaster unravels lesion-specific expression blueprints across muscle mutations
| Autor: | Mohammed Aiyaz, Upendra Nongthomba, Prabodh Kumar, Divesh Thimmaiya, Aditi Madan, John C. Sparrow, Ari Franco-Cea |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Muscle tissue Scaffold protein Male Biology Protein degradation Myosins Sarcomere 03 medical and health sciences 0302 clinical medicine Myosin Genetics medicine Animals Drosophila Proteins Myopathy Actin Oligonucleotide Array Sequence Analysis Gene Expression Profiling General Medicine Actins Muscle Striated Cell biology 030104 developmental biology medicine.anatomical_structure Mutation Drosophila medicine.symptom 030217 neurology & neurosurgery Muscle contraction Muscle Contraction |
| Zdroj: | Gene. 631 |
| ISSN: | 1879-0038 |
| Popis: | Muscle contraction is a highly fine-tuned process that requires the precise and timely construction of large protein sub-assemblies to form sarcomeres. Mutations in many genes encoding constituent proteins of this macromolecular machine result in defective functioning of the muscle tissue. However, the pathways underlying muscle degeneration, and manifestation of myopathy phenotypes are not well understood. In this study, we explored transcriptional alterations that ensue from the absence of the two major muscle proteins - myosin and actin - using the Drosophila indirect flight muscles. Our aim was to understand how the muscle tissue responds as a whole to the absence of either of the major scaffold proteins, whether the responses are generic to the tissue; or unique to the thick versus thin filament systems. Our results indicated that muscles respond by altering gene transcriptional levels in multiple systems active in muscle remodelling, protein degradation and heat shock responses. However, there were some responses that were filament-specific signatures of muscle degeneration, like immune responses, metabolic alterations and alterations in expression of muscle structural genes and mitochondrial ribosomal genes. These general and filament-specific changes in gene expression may be of relevance to human myopathies. |
| Databáze: | OpenAIRE |
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