The construction of DNA molecules of figure-eight structure
| Autor: | Hadar Nir, Gadi Schuster, Yoav Eichen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Stereochemistry
Biophysics DNA Single-Stranded Nanotechnology Microscopy Atomic Force Biochemistry Restriction fragment Bacteriophage chemistry.chemical_compound Nucleic acid thermodynamics Plasmid Molecular Biology Gel electrophoresis biology Nucleic Acid Hybridization DNA DNA Restriction Enzymes Cell Biology biology.organism_classification Restriction enzyme Oligodeoxyribonucleotides Complementary sequences chemistry DNA Viral biology.protein Nucleic Acid Conformation DNA Circular Bacteriophage M13 Plasmids |
| Zdroj: | Analytical Biochemistry. 344:86-91 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2005.06.029 |
| Popis: | Using DNA molecules to construct a structural scaffold for nanotechnology is largely accepted. In this article, we report on two methods for constructing a figure-eight structure of DNA molecules having a relatively high yield that could be used further as a scaffold for nanotechnology applications. In the first method, two plasmids were constructed that, on digestion with a restriction endonuclease producing nicks in the corresponding sites and after heating, produced complementary single-stranded sequences, enabling the plasmids to hybridize to each other and forming a figure-eight structure. The formation of the figure-eight structure was analyzed by restriction analysis and gel electrophoresis as well as by atomic force microscopy. The second method makes use of the bacteriophage M13 that is obtained as either a single- or double-stranded circular DNA molecule. Two M13 molecules harboring complementary sequences were constructed and produced a figure-eight structure on hybridization. The methods described here could be used further for the construction of nanoelectronic devices. |
| Databáze: | OpenAIRE |
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