A simple method for preparation of d-rhamnose
| Autor: | Michael Ramm, Martina Lobe, Matthias Hamburger |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Lipopolysaccharides
chemistry.chemical_classification Chromatography Rhamnose Hydrolysis Hydrophilic interaction chromatography Organic Chemistry Phenol extraction General Medicine Cleavage (embryo) Biochemistry Analytical Chemistry chemistry.chemical_compound Column chromatography chemistry Pseudomonas Pseudomonas syringae Monosaccharide |
| Zdroj: | Carbohydrate Research. 338:109-112 |
| ISSN: | 0008-6215 |
| DOI: | 10.1016/s0008-6215(02)00353-1 |
| Popis: | A rapid procedure for the preparation of D-rhamnose from bacterial lipopolysaccharide (LPS) has been developed. It involves purification of LPS from Pseudomonas syringae pv. phaseolicola by phenol extraction and hydrophobic interaction chromatography (HIC), followed by mild hydrolysis and cleavage of the O-antigen into D-fucose and D-rhamnose. The monosaccharides were separated by column chromatography, and D-rhamnose recovered after filtration over Sephadex-LH 20. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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