Measurement of Streptococcal Antigen Synthesis with Fluorescent Antibody
| Autor: | Eugene N. Fox |
|---|---|
| Rok vydání: | 1962 |
| Předmět: |
Antigens
Bacterial Globulin biology Chemistry Extraction (chemistry) Cell Fluorescent Antibody Technique Streptococcus Molecular biology Fluorescence General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound medicine.anatomical_structure Antigen biology.protein medicine Somatic antigen Antigens Fluorescein Antibody |
| Zdroj: | Experimental Biology and Medicine. 109:577-579 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | Summary and conclusionThe amount of specific antibody adsorbed by streptococci during the process of de novo somatic antigen synthesis was a function of the amount of antigen on the cell. The fluorescent technic when compared with acid extraction demonstrated that considerably fewer cells were required to observe the progress of synthesis and, in addition, there was obtained a value more quantitative than the estimation of the degree of precipitation in capillary tubes.Controls with fluorescein-conjugated normal globulin indicated the degree of nonspecific binding of conjugated protein by the streptococcal cells. The basal limit of nonspecific adsorption was considerably higher when fluorescein-labeled whole sera were employed rather than the globulin fractions.Standard curves obtained with buffered fluorescein solutions were linear within the total range of the instrument. These values were between 0.02 and 2.0 μg of fluorescein per ml. The fluorescent values of Fig. 1 were within a range of 0.04 to 0.25... |
| Databáze: | OpenAIRE |
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