Fatty acid composition of brain capillary endothelial cells: effect of the coculture with astrocytes
| Autor: | Michel Lagarde, M P Dehouck, R Cecchelli, C Bénistant, J.-C. Fruchart |
|---|---|
| Přispěvatelé: | Centre de recherches de biochimie macromoléculaire (CRBM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-IFR122-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), Régulations métaboliques, nutrition et diabètes (RMND), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL) |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Endothelium
Linoleic acid QD415-436 Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Phosphatidylcholine Free fatty acid receptor 1 medicine [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology n-6 fatty acids Phospholipids 030304 developmental biology Blood-brain barrier chemistry.chemical_classification 0303 health sciences Fatty acid Cell Biology n-3 fatty acids Endothelial stem cell medicine.anatomical_structure chemistry Docosahexaenoic acid Arachidonic acid 030217 neurology & neurosurgery |
| Zdroj: | Journal of Lipid Research, Vol 36, Iss 11, Pp 2311-2319 (1995) The Journal of Lipid Research The Journal of Lipid Research, 1995, 36, p. 2311-2319 Journal of Lipid Research Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 1995, 36, p. 2311-2319 |
| ISSN: | 0022-2275 |
| Popis: | International audience; We have investigated the fatty acid composition of brain capillary endothelial cells cultured alone or in coculture with astrocytes, using an in vitro model in which endothelial cells and astrocytes were grown from one part of a filter to another. We found that the fatty acid composition of the cocultured cerebral endothelial cells was markedly different from that of non-cocultivated endothelial cells. The most striking difference was the increase of arachidonic acid (20:4n-6) at the expense of its precursor, linoleic acid (18:2n-6). Similar modifications were found for the n-3 family of fatty acids with an increase of docosahexaenoic acid (22:6n-3) at the expense of its precursors, but the differences were less than within the n-6 fatty acids. These changes induced by the coculture were observed only in endothelial cell phospholipids, especially the phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine classes, but were not detected in phosphatidylinositols and in other lipid classes. Only the composition of the n-3 series fatty acids was altered in another capillary endothelial cell type (from adrenal cortex) cocultured with astrocytes under the same conditions. The fatty acid changes observed might be biologically relevant as they tended to make the fatty acid composition of the brain capillary endothelial cells more closely resemble that of brain microvessels. |
| Databáze: | OpenAIRE |
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