Isolation of nuclei from red and white skeletal muscles of the adult rat
| Autor: | R.T. Rodrigo, H.C. Yeoh, H. Tonaki, Irene R. Held |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Male
Muscle Proteins In Vitro Techniques Biology Cell Fractionation law.invention Protein content chemistry.chemical_compound law RNA polymerase medicine Animals Microscopy Phase-Contrast Cell Nucleus Muscles RNA DNA DNA-Directed RNA Polymerases Cell Biology Molecular biology Rats White (mutation) Microscopy Electron Cell nucleus medicine.anatomical_structure chemistry Cell fractionation Electron microscope |
| Zdroj: | Experimental Cell Research. 105:191-197 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(77)90165-3 |
| Popis: | The distinct procedures required for the isolation of nuclei from red and white skeletal muscles of the adult rat in reproducible yields of 15 and 20%, respectively, are described. The purity and preservation of the isolated nuclei were evaluated by phase contrast and electron microscopy, by analyses of RNA and protein content and by RNA polymerase assays. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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