Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
| Autor: | María Andrea Crepy, Ruth Amelia Heinz, Paula Fernández, Gustavo Gabriel Arguissain, Martha Lucrecia Bonell |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biplot Introgression Plant Science Horticulture Quantitative trait locus 01 natural sciences CANDIDATE GENES COLD TOLERANCE AT SEEDLING STAGE (CTS) 03 medical and health sciences COLD TOLERANCE (CT) Genotype Genetics Cultivar Allele Oryza sativa biology MARKER-ASSISTED SELECTION (MAS) LOW-TEMPERATURE GERMINATION ABILITY (LTG) food and beverages purl.org/becyt/ford/4.4 [https] biology.organism_classification nervous system diseases 030104 developmental biology Seedling Agronomy and Crop Science purl.org/becyt/ford/4 [https] 010606 plant biology & botany |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| DOI: | 10.1007/s10681-020-02661-3 |
| Popis: | In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina |
| Databáze: | OpenAIRE |
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