SERF: a new antigen in the Cromer blood group system
| Autor: | P. Palacajornsuk, J. Banks, B-W Ma, A Seltsam, Kim Hue-Roye, Jill R. Storry, Norbert Ahrens, Abdulgabar Salama, Douglas M. Lublin, Joyce Poole, M. E. Reid |
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| Jazyk: | němčina |
| Rok vydání: | 2004 |
| Předmět: |
Proband
Genetics Isoantigens ddc:610 Erythrocytes Base Sequence Reverse Transcriptase Polymerase Chain Reaction Sequence analysis Point mutation CD55 Cromer blood group decay-accelerating factor high incidence antigen SERF 610 Medizin Hematology Biology Molecular biology Epitope Exon Antigen Complementary DNA Blood Group Antigens Humans Decay-accelerating factor Polymorphism Restriction Fragment Length DNA Primers |
| Popis: | The Cromer blood group system consists of eight high incidence and three low incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high incidence antigen, named SERF. Sequence analyses of DNA from a Thai female whose serum contained the alloantibody to a high incidence antigen in the Cromer blood group system (anti-SERF) and from her two children were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis on cDNA from the proband and PCR-restriction fragment length polymorphism analysis on DNA from Thais were also performed. To map the epitope, DAF deletion mutants were tested by immunoblotting with anti-SERF. Sequence analysis revealed a substitution of 647C>T in exon 5 DAF in the proband. The proband's two children and two of 100 Thais were heterozygotes 647C/T. Analysis using DAF deletion mutants revealed the antigenic determinant to be within short consensus repeat 3 (SCR3), which is encoded by exon 5. This study describes a novel high incidence antigen (SERF) in the Cromer blood group system characterized by the amino acid proline at position 182 in SCR3 of DAF. The SERF-negative proband has a substitution mutation that predicts for leucine at this position. SERF has been provisionally assigned the International Society of Blood Transfusion number 021��012 (CROM 12). |
| Databáze: | OpenAIRE |
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