Comparison of Enzymatic Properties of DNA‐Abzymes and Human DNAses

Autor:	Georgy A. Nevinsky, E. S. Odintsova, B. M. Doronin, V. N. Buneva, A. G. Baranovskii
Rok vydání:	2004
Předmět:	Multiple Sclerosis Antibodies Catalytic Biochemistry Substrate Specificity chemistry.chemical_compound Genetics medicine Humans Lupus Erythematosus Systemic Biochemical markers chemistry.chemical_classification Deoxyribonucleases Base Sequence biology Hydrolysis Multiple sclerosis General Medicine medicine.disease Molecular biology Abzyme Enzyme chemistry Polyclonal antibodies biology.protein Molecular Medicine Electrophoresis Polyacrylamide Gel Antibody DNA
Zdroj:	Nucleosides, Nucleotides and Nucleic Acids. 23:1053-1056
ISSN:	1532-2335 1525-7770
Popis:	DNA-hydrolyzing antibodies (DNA-abzymes, Abz) were shown to be good biochemical markers of some autoimmune diseases such as systemic lupus erythematosus (SLE) and multiple sclerosis (MS). To better understand mechanisms of abzyme generation, one needs to know optimal conditions for DNA hydrolysis by DNA-abzymes, as well as their enzymatic properties in comparison with those of enzymes possessing the same activity. In contrast to human urine deoxyribonucleases, DNA-hydrolyzing antibodies efficiently digested both single- and double-strand DNA. It was shown that polyclonal antibodies (Abs) in MS may contain up to several types of DNase activities, either activated by metal ions or not.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec628698915f48619a659658bfd5867b https://doi.org/10.1081/ncn-200026068 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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