Steroidogenic factor 1 is the essential transcript of the mouse Ftz-F1 gene
| Autor: | Keith L. Parker, Xunrong Luo, David A. Schlosser, Yayoi Ikeda |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Steroidogenic factor 1 medicine.medical_specialty Sex Differentiation medicine.medical_treatment Period (gene) Fushi Tarazu Transcription Factors Receptors Cytoplasmic and Nuclear Biology Steroidogenic Factor 1 Transfection Mice Structure-Activity Relationship Methionine Endocrinology Adrenal Cortex Hormones Internal medicine Adrenal Glands medicine Animals Molecular Biology Gene Homeodomain Proteins Mice Knockout Sexual differentiation fungi Genitalia Female General Medicine Phenotype Pedigree DNA-Binding Proteins Steroid hormone Mutation Knockout mouse Mutagenesis Site-Directed Female Glucocorticoid Transcription Factors medicine.drug |
| Zdroj: | Molecular Endocrinology. 9:1233-1239 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | Targeted disruption of the mouse Ftz-F1 gene, which encodes the orphan nuclear receptors steroidogenic factor 1 (SF-1) and embryonal long terminal repeat-binding protein (ELP), established that this gene is essential for development of the primary steroidogenic tissues and for male sexual differentiation. Associated with these dramatic developmental abnormalities, all Ftz-F1-disrupted mice died in the immediate postnatal period and had very low glucocorticoid levels. In this report, we show that treatment with corticosteroids markedly prolonged survival of the Ftz-F1-disrupted mice, proving that steroid hormone deficiency causes their death. We also generated SF-1-specific knockout mice with a targeting construct that specifically disrupted the SF-1 coding sequence without impairing the ELP protein. The phenotype of the SF-1-specific knockout mice was indistinguishable from that observed in Ftz-F1-disrupted mice that lack both SF-1 and ELP. Taken together, these results indicate that SF-1 is the Ftz-F1-encoded protein that is required for multiple aspects of endocrine development and for postnatal survival. |
| Databáze: | OpenAIRE |
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