Characterization by gas--liquid chromatography--mass spectrometry of oligosaccharides resulting from the hydrazinolysis-nitrous acid deamination reaction of glycopeptides
| Autor: | Annick Pierce-Cretel, Bernard Fournet, Jean Montreuil, Gérard Strecker, Geneviève Spik |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Chemical Phenomena
Biophysics Deamination Oligosaccharides Nitrous Acid Mass spectrometry Cleavage (embryo) Biochemistry Gas Chromatography-Mass Spectrometry Sodium borohydride chemistry.chemical_compound Present method Organic chemistry Humans Molecular Biology Nitrous acid Chromatography Milk Human Chemistry Glycopeptides Cell Biology Glycopeptide Hydrazines Female Gas chromatography |
| Zdroj: | Analytical biochemistry. 111(1) |
| ISSN: | 0003-2697 |
| Popis: | The hydrazinolysis-nitrous acid deamination of glycopeptides leads to the specific cleavage of N-acetylglucosamine linkages. The 2,5-anhydro-mannose containing oligosaccharides obtained by this way are reduced by sodium borohydride, methylated, and analyzed by gas-liquid chromatography- mass spectrometry. Here we report the characterization of 11 oligosaccharides obtained from various fucosyl-glycoasparagines and glycopeptides. In addition, the structure of a partially characterized glycopeptide isolated from human milk sIgA was investigated as an application of the present method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|