Evaluation of eight preservation solutions for endothelial in situ preservation
| Autor: | Anne E. Cunningham, Colin H Wilson, Malcolm Haswell, Gerard Stansby, David Talbot |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Adenosine Endothelium medicine.medical_treatment Allopurinol Organ Preservation Solutions Biology Nitric oxide Andrology chemistry.chemical_compound Raffinose medicine Animals Insulin Viaspan Saline Vascular tissue Transplantation Tissue Preservation Glutathione Surgery Rats Endothelial stem cell medicine.anatomical_structure chemistry Rats Inbred Lew Models Animal Tonicity Endothelium Vascular |
| Zdroj: | Transplantation. 78(7) |
| ISSN: | 0041-1337 |
| Popis: | Background. Non-heart-beating donors (NHBDs) have the potential to reduce the increasing numbers of patients on kidney and liver graft waiting lists. One problem observed with kidneys obtained from NHBDs is the endothelial injury seen on protocol core biopsies after implantation. We postulate that this is caused by a combination of warm ischemia, cold ischemia, and hypertonic citrate during in situ preservation (ISP) rather than hypothermic machine preservation. Our aim was to optimize ISP methods to preserve endothelial structure and function. Methods. An animal model of ISP was used to compare the ability of eight different preservation solutions to protect mammalian vascular tissue exposed to a combination of warm and cold ischemia. Smooth muscle contractile function and endothelial dependent relaxation (nitric oxide production) were determined using an organ bath method. Results. Bretchneider's HTK solution preserved the ability of endothelial tissue to relax vascular tissue in response to acetylcholine (91% relaxation vs. 17% saline control; ANOVA, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|