Antitumor and Radiosensitizing Effects of Zinc Oxide-Caffeic Acid Nanoparticles against Solid Ehrlich Carcinoma in Female Mice
| Autor: | Nadia Y.S. Morcos, Mahmoud M. Said, Amel F.M. Ismail, Hayam M Sayed, Mona A El Gawish |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Radiation-Sensitizing Agents Population chemistry.chemical_element Zinc 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Caffeic Acids In vivo Spectroscopy Fourier Transform Infrared Caffeic acid Animals Viability assay education female mice RC254-282 antitumor education.field_of_study Chemistry Cell adhesion molecule Carcinoma radiosensitizing effect Neoplasms. Tumors. Oncology. Including cancer and carcinogens Molecular biology solid Ehrlich carcinoma In vitro Zinc Oxide-Caffeic Acid Nanoparticles (ZnO-CA NPs) 030104 developmental biology Complementary and alternative medicine Oncology 030220 oncology & carcinogenesis DNA fragmentation Nanoparticles Female Zinc Oxide Research Article |
| Zdroj: | Integrative Cancer Therapies, Vol 20 (2021) Integrative Cancer Therapies |
| ISSN: | 1534-7354 |
| Popis: | This study aimed to evaluate the anticancer and radio-sensitizing efficacy of Zinc Oxide-Caffeic Acid Nanoparticles (ZnO-CA NPs). ZnO-CA NPs were formulated by the conjugation of Zinc Oxide nanoparticles (ZnO NPs) with caffeic acid (CA) that were characterized by Fourier Transform Infrared Spectra (FT-IR), X-ray Diffractometer (XRD), and Transmission Electron Microscopy (TEM). In vitro anticancer potential of ZnO-CA NPs was evaluated by assessing cell viability in the human breast (MCF-7) and hepatocellular (HepG2) carcinoma cell lines. In vivo anticancer and radio-sensitizing effects of ZnO-CA NPs in solid Ehrlich carcinoma-bearing mice (EC mice) were also assessed. Treatment of EC mice with ZnO-CA NPs resulted in a considerable decline in tumor size and weight, down-regulation of B-cell lymphoma 2 (BCL2) and nuclear factor kappa B (NF-κB) gene expressions, decreased vascular cell adhesion molecule 1 (VCAM-1) level, downregulation of phosphorylated-extracellular-regulated kinase 1 and 2 (p-ERK1/2) protein expression, DNA fragmentation and a recognizable peak at sub-G0/G1 indicating dead cells’ population in cancer tissues. Combined treatment of ZnO-CA NPs with γ-irradiation improved these effects. In conclusion: ZnO-CA NPs exhibit in-vitro as well as in-vivo antitumor activity, which is augmented by exposure of mice to γ-irradiation. Further explorations are warranted previous to clinical application of ZnO-CA NPs. |
| Databáze: | OpenAIRE |
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