ULTRASTRUCTURAL LOCALIZATION OF CONTRACTILE PROTEIN (THROMBOSTHENIN) IN HUMAN PLATELETS USING AN UNLABELED ANTIBODY-PEROXIDASE STAINING TECHNIQUE
| Autor: | Dorothea Zschocke, Max E. Rafelson, Francois M. Booyse, Ludwig A. Sternberger |
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| Rok vydání: | 1971 |
| Předmět: |
Blood Platelets
Cytoplasm Histology Pronase Antigen-Antibody Reactions Platelet Adhesiveness Antibody Specificity Methods Animals Humans Platelet Immunoelectrophoresis Incubation Plant Proteins Antiserum Sheep Staining and Labeling biology Histocytochemistry Immune Sera Immunochemistry Cell Membrane Blood Proteins Blood Protein Electrophoresis Molecular biology Microscopy Electron Peroxidases Biochemistry Immunoglobulin G biology.protein Ultrastructure Rabbits Anatomy Antibody Peroxidase |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 19:540-550 |
| ISSN: | 1551-5044 0022-1554 |
| DOI: | 10.1177/19.9.540 |
| Popis: | Soluble horseradish peroxidase-antihorseradish peroxidase (rabbit) complex was used to localize the actomyosin-like contractile protein, thrombosthenin, in both intact human platelets (Epon-embedded) and ultrathin sections (methacrylate-embedded). Antibody staining of ultrathin sections showed the presence of membrane-associated and cytoplasmic thrombosthenin. Antibody staining of intact cells showed that membrane-associated thrombosthenin was localized, at least in part, in the exterior, "fluffy" coat of the platelet. Even after prolonged fixation and/or incubation with the various antisera, we were unable to demonstrate antibody penetration of intact, fixed platelets. Brief treatment with Pronase removed the surface-localized proteinaceous material and completely abolished all antibody staining. |
| Databáze: | OpenAIRE |
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