Histological Staining with two Direct Textile Dyes: Pontamine Black Rrx and Pontamine Fast Black Pgr
| Autor: | Anthony E. Bedrick |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Cell Nucleus
Cytoplasm Pathology medicine.medical_specialty Blood Cells Hyperplasia Aqueous solution Staining and Labeling Chemistry Mouth Mucosa Oral mucous membrane Hydrogen-Ion Concentration Staining Fibrous hyperplasia Histological staining Connective Tissue Paraffin section Carcinoma Squamous Cell medicine Humans Mouth Neoplasms Basal cell Anatomy Coloring Agents Azo Compounds |
| Zdroj: | Stain Technology. 41:335-337 |
| ISSN: | 0038-9153 |
| DOI: | 10.3109/10520296609116336 |
| Popis: | In paraffin sections of formalin-fixed human oral mucous membrane, Pontamine black RRX (Direct Black 5, C. I. 30245) stained both squamous cell carcinoma and benign fibrous hyperplasia. A 2% solution of the former (PB RRX) required a 20 min staining time; a 3% solution of the latter (PFB PGR), 30 min. Staining was tested for acidified, unmodified, and alkalinized aqueous solutions of the dyes. The addition of 1 ml of concentrated NH, OH to 100 ml of the PB RRX improved staining slightly; acidification reduced the intensity. The addition of 4 ml of 1 N HCI to 100 ml of the PFB PGR enhanced the speed and intensity of staining; alkalinization was without effect. |
| Databáze: | OpenAIRE |
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