Network Pharmacological Screening of Herbal Monomers that Regulate Apoptosis-Associated Genes in Acute Pancreatitis
| Autor: | Bo Gao, Shaoyue Zheng, Weihui Zhang, Yanfeng Jiang, Ming Lu, Dongbo Xue, Bei Sun, Xin Qiao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Gene regulatory network Apoptosis Computational biology Bioinformatics 03 medical and health sciences 0302 clinical medicine Endocrinology Gene expression Databases Genetic Drug Discovery Internal Medicine Medicine Humans Gene Regulatory Networks Protein Interaction Maps Human Protein Reference Database Gene Regulation of gene expression Hepatology business.industry Drug discovery Plant Extracts Gene Expression Profiling Computational Biology Gene expression profiling 030104 developmental biology Gene Ontology Gene Expression Regulation Pancreatitis 030220 oncology & carcinogenesis Acute Disease business Function (biology) Software Phytotherapy |
| Zdroj: | Pancreas. 46(1) |
| ISSN: | 1536-4828 |
| Popis: | Objectives In this study, we screened for differentially expressed genes in acute pancreatitis and the herbal monomers that regulate these genes. Methods Gene expression profile data were downloaded from the Gene Expression Omnibus database (GSE3644). We used the Human Protein Reference Database to determine the protein-protein interaction network and CFinder software (Department of Biological Physics of Eotvos University, Budapest, Hungary) to identify several functional modules. Then, we used Database for Annotation, Visualization and Integrated Discovery software (Frederick, Md) to perform a gene ontology-biological process functional enrichment analysis. Based on a database of herbal monomers and a literature search, we constructed a gene-herbal monomer regulatory network using Cytoscape software (San Diego, Calif), and we analyzed the relationships between apoptosis, genes, and herbal monomers. Results A total of 1745 differentially expressed genes were identified. Nine modules were identified, and the main function of module 3 was closely related to apoptosis. Within module 3, we selected 13 genes that were closely related to apoptosis for further analysis. In the gene-herbal monomer regulatory network, 18 herbal monomers that regulate multiple target genes were selected as the focus of this study. Conclusions These herbal monomers regulate multiple target genes to induce apoptosis and may potentially be used as new drugs for acute pancreatitis treatment in the future. |
| Databáze: | OpenAIRE |
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