Medakaeyelessis the key factor linking retinal determination and eye growth
| Autor: | Detlev Arendt, Clemens Grabher, Matthias Carl, Sylke Winkler, Thorsten Henrich, Joachim Wittbrodt, Wilhelm Ansorge, Elisa Wurmbach, Erika Grzebisz, Felix Loosli, Carola Burgtorf, Annette Krone |
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| Rok vydání: | 2001 |
| Předmět: |
Fish Proteins
DNA Complementary PAX6 Transcription Factor Molecular Sequence Data Oryzias Morphogenesis Nerve Tissue Proteins Locus (genetics) Biology Eye Retina medicine Animals Drosophila Proteins Paired Box Transcription Factors Amino Acid Sequence Eye Proteins Molecular Biology Homeodomain Proteins Genetics Base Sequence Genes Homeobox Temperature Gene Expression Regulation Developmental Optic vesicle Phenotype eye diseases Cell biology DNA-Binding Proteins Repressor Proteins medicine.anatomical_structure Evagination Mutation Homeobox sense organs PAX6 T-Box Domain Proteins Developmental Biology |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.128.20.4035 |
| Popis: | The complete absence of eyes in the medaka fish mutation eyeless is the result of defective optic vesicle evagination. We show that the eyeless mutation is caused by an intronic insertion in the Rx3 homeobox gene resulting in a transcriptional repression of the locus that is rescued by injection of plasmid DNA containing the wild-type locus. Functional analysis reveals that Six3- and Pax6- dependent retina determination does not require Rx3. However, gain- and loss-of-function phenotypes show that Rx3 is indispensable to initiate optic vesicle evagination and to control vesicle proliferation, by that regulating organ size. Thus, Rx3 acts at a key position coupling the determination with subsequent morphogenesis and differentiation of the developing eye. |
| Databáze: | OpenAIRE |
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