Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo
Autor: | Sandu Pitaru, Alexander D. Vardimon, Sandra Treves-Manusevitz, Higinio Arzate, A. Sampath Narayanan, Gonzalo Montoya, Lía Hoz, Ephraim Tzur, Heled Rachima, Salomon Amar |
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Rok vydání: | 2012 |
Předmět: |
Mineralized tissues
Bone sialoprotein Pathology medicine.medical_specialty Bone Regeneration Osteocalcin Gingiva Mice SCID Mice medicine Integrin-Binding Sialoprotein Animals Humans Regeneration Cementum Oral mucosa Bone regeneration Dental Cementum Fibrin Lamina propria Amelogenin biology Chemistry Stem Cells Mouth Mucosa Proteins Alkaline Phosphatase medicine.anatomical_structure biology.protein Periodontics Collagen Dental cementum Protein Tyrosine Phosphatases Stem Cell Transplantation |
Zdroj: | Journal of Clinical Periodontology. 40:73-81 |
ISSN: | 0303-6979 |
Popis: | Aims To characterize the mineralized tissue formed constitutively in the supracalvarial region of scid mice by a primitive stem cell population (hOMSC) derived from the lamina propria of the human oral mucosa and gingiva. Material and Methods Fibrin–hOMSC constructs were cultured for 14 days at which time point they were analysed for the expression of osteoblastic/cementoblastic markers and implanted between the skin and calvaria bones into scid mice. After 8 weeks, the animals were sacrificed and the implantation sites analysed. Results Two-week-old cultures of fibrin–hOMSC constructs expressed osteogenic/cementogenic markers at the gene level. Macroscopic and radiographic examinations revealed mineralized masses at the implantation sites of fibrin–hOMSC constructs. Histology, histochemistry and immunofluorescence showed mineralized masses consisting of avascular cellular and acellular matrices that stained positively for collagen, Ca, cementum attachment protein, cementum protein 1, bone sialoprotein, alkaline phosphatase, osteocalcin, amelogenin and ameloblastin. Positive anti-human nuclear antigen indicated the human origin of the cells. Atomic force microscopy depicted long prismatic structures organized in lamellar aggregates. Conclusions Within the limitation of this study, the results indicate for the first time that fibrin–hOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone. |
Databáze: | OpenAIRE |
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