Role of mRNA stability during genome-wide adaptation of Lactococcus lactis to carbon starvation
| Autor: | Emma Redon, Pascal Loubière, Muriel Cocaign-Bousquet |
|---|---|
| Přispěvatelé: | Unité mixte de recherche biotechnologies bioprocédés, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2005 |
| Předmět: |
Exonuclease
Exonucleases RNA Stability DNA Complementary Time Factors Transcription Genetic adaptation Biochemistry 03 medical and health sciences Open Reading Frames STABILITÉ privation de carbone Transcription (biology) Gene expression RNA Messenger [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology Gene 030304 developmental biology bactérie 2. Zero hunger Regulation of gene expression 0303 health sciences biology 030306 microbiology gène Lactococcus lactis conduite de la culture Computational Biology Nucleic Acid Hybridization Cell Biology Gene Expression Regulation Bacterial biology.organism_classification Carbon Open reading frame Purines biology.protein RNA LACTOCOCCUS LACTIS arn messager Genome Bacterial |
| Zdroj: | Journal of Biological Chemistry 43 (280), 36380-36385. (2005) Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2005, 280 (43), pp.36380-36385. ⟨10.1074/jbc.M506006200⟩ Journal of Biological Chemistry, 2005, 280 (43), pp.36380-36385. ⟨10.1074/jbc.M506006200⟩ |
| ISSN: | 0021-9258 1083-351X |
| Popis: | International audience; The stability of mRNA was investigated for the first time at the genomic scale during carbon starvation adaptation of Lactococcus lactis IL1403. In exponential phase, mRNA half-lives were correlated positively to open reading frame length. A polypurine sequence, AGGAG, was identified as a putative 5′-stabilizer and inverted repeated sequences as a 3′-destabilizer. These original findings suggested that multiple pathways of mRNA degradation should coexist: internal cleavage, endonuclease cleavage initiated at the 5′-end, and exonuclease attack at the 3′-end. During carbon starvation adaptation, mRNA stability globally increased, but specific mechanisms allowing a wide range of stabilization factors between genes and differential kinetic evolution were involved. A formal method allowing the quantification of the relative influences of transcription and degradation on the mRNA pool control was developed and applied in L. lactis. Gene expression was mostly controlled by altered transcription prior to carbon source exhaustion, while the influence of mRNA stability increased during the starvation phase. This study highlighted that stability modulation in response to adverse growth conditions can govern gene regulation to the same extent as transcription in bacteria. |
| Databáze: | OpenAIRE |
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