Direct Testing for KPC-Mediated Carbapenem Resistance from Blood Samples Using a T2 Magnetic Resonance Based Assay
| Autor: | Maurizio Sanguinetti, Antonella Mencacci, Riccardo Paggi, Brunella Posteraro, Giulia Menchinelli, Jessica L. Snyder, Giulia De Angelis, Thomas Jay Lowery |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
KPC carbapenemase bloodstream infection RM1-950 Biochemistry Microbiology Settore MED/07 - MICROBIOLOGIA E MICROBIOLOGIA CLINICA blood sample magnetic resonance T2Resistance panel Antibiotic resistance Bloodstream infection Antibiotic therapy polycyclic compounds Medicine Pharmacology (medical) antimicrobial resistance General Pharmacology Toxicology and Pharmaceutics Gene Carbapenem resistance biology business.industry Brief Report biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Citrobacter freundii Infectious Diseases Positive blood culture direct detection Therapeutics. Pharmacology Carbapenem resistance genes business |
| Zdroj: | Antibiotics, Vol 10, Iss 950, p 950 (2021) Antibiotics |
| ISSN: | 2079-6382 |
| Popis: | Molecular-based carbapenem resistance testing in Gram-negative bacterial bloodstream infections (BSIs) is currently limited because of the reliance on positive blood culture (BC) samples. The T2Resistance™ panel may now allow the detection of carbapenemase- and other β-lactamase encoding genes directly from blood samples. We detected carbapenem resistance genes in 11 (84.6%) of 13 samples from patients with BC-documented BSIs (10 caused by KPC-producing Klebsiellapneumoniae and 1 caused by VIM/CMY-producing Citrobacter freundii). Two samples that tested negative for carbapenem resistance genes were from patients with BC-documented BSIs caused by KPC-producing K. pneumoniae who were receiving effective antibiotic therapy. In conclusion, our findings suggest that the T2Resistance™ panel can be a reliable tool for diagnosing carbapenem-resistant Gram-negative bacterial BSIs. |
| Databáze: | OpenAIRE |
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