Transcriptomic analysis of chicken cochleae after gentamicin damage and the involvement of four signaling pathways (Notch, FGF, Wnt and BMP) in hair cell regeneration
| Autor: | Xinwen Zhang, Xuebo Zhang, Lingling Jiang, Huanju Bai, Meiguang Zhang, Siyuan Yang, Jincao Xu, Ran Jin, Zhongming Han, Shaoju Zeng |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biology Fibroblast growth factor Transcriptome Tissue Culture Techniques 03 medical and health sciences Hair Cells Auditory medicine Animals Regeneration Mitosis Hearing Disorders Cochlea Cell Proliferation Receptors Notch Regeneration (biology) Gene Expression Profiling Wnt signaling pathway Cell Differentiation Sensory Systems Cell biology Fibroblast Growth Factors Wnt Proteins Disease Models Animal 030104 developmental biology medicine.anatomical_structure Animals Newborn Bone Morphogenetic Proteins Hair cell Signal transduction Gentamicins Chickens Signal Transduction |
| Zdroj: | Hearing research. 361 |
| ISSN: | 1878-5891 |
| Popis: | Unlike mammalian hair cells, which are essentially unable to regenerate after damage, avian hair cells have a robust capacity for regeneration. The prerequisite for understanding the above difference is knowing the genetic programming of avian hair cell regeneration. Although the major processes have been known, the precise molecular signaling that induces regeneration remains unclear. To address this issue, we performed a high-throughput transcriptomic analysis of gene expression during hair cell regeneration in the chick cochlea after antibiotic injury in vivo. A total of 16,588 genes were found to be expressed in the cochlea, of which about 1000 genes were differentially expressed among the four groups studied, i.e., 2 days (d) or 3 d post-treatment with gentamicin or physiological saline. The differentially expressed genes were distributed across approximately one hundred signaling pathways, including the Notch, MAPK (FGF), Wnt and TGF-β (BMP) pathways that have been shown to play important roles in embryonic development. Some differentially expressed genes (2–3 in each pathway) were further verified by qRT-PCR. After blocking Notch, FGF or BMP signaling, the number of regenerating hair cells and mitotic supporting cells increased. However, the opposite effect was observed after suppressing the Wnt pathway or enhancing BMP signaling. To our knowledge, the present study provided a relatively complete dataset of candidate genes and signaling pathways most likely involved in hair cell regeneration and should be a useful start in deciphering the genetic circuitry for inducing hair cell regeneration in the chick cochlea. |
| Databáze: | OpenAIRE |
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