Characterization of 10 tobacco vein banding mosaic virus isolates from China
| Autor: | K. Li, Q. Han, Ch. Zhu, T. Yuan, F. Wen, Y. Song |
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| Rok vydání: | 2012 |
| Předmět: |
China
Population Molecular Sequence Data Genome Viral Biology Genome Virology Complementary DNA Tobacco Cloning Molecular Selection Genetic education Gene 3' Untranslated Regions Phylogeny Plant Diseases Whole genome sequencing Recombination Genetic education.field_of_study Phylogenetic tree Base Sequence Nucleic acid sequence RNA General Medicine Sequence Analysis DNA Tobacco Mosaic Virus Infectious Diseases RNA Viral |
| Zdroj: | Acta virologica. 56(1) |
| ISSN: | 0001-723X |
| Popis: | The complete RNA genome sequences of 10 tobacco vein banding mosaic virus (TVBMV) isolates from China were determined using five overlapping cDNA clones. TVBMV was divided into three groups for HC-Pro and CP and into two groups for P3 and 6K1. With more isolate sequences analyzed, the phylogenetic results suggest that TVBMV could be divided into four subgroups based on HC-Pro and CP and into three subgroups with P3 and 6K1. Nucleotide sequence diversity analysis showed geographical differentiation among the TVBMV isolates. Three of the 10 isolates were found to have undergone recombination and new recombination sites were identified in the TVBMV genome. All coding genes were under negative selection and the population appeared to have remained stable over a long period. This study also provides preliminary data on the 3´-untranslated region as potentially the best genome sequence for developing transgenic tobacco. |
| Databáze: | OpenAIRE |
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