Cutting Edge: Receptor-Mediated Endocytosis of Heat Shock Proteins by Professional Antigen-Presenting Cells

Autor:	Arnold-Schild, D., Hanau, D., Spehner, D., Schmid, C., Rammensee, H. G., Henri de la Salle, Schild, H.
Rok vydání:	1999
Předmět:	Leukemia P388 Immunology H-2 Antigens Receptors Antigen T-Cell Antigen-Presenting Cells Coated Pits Cell-Membrane Endosomes Clathrin Endocytosis Cell Line Mice Antigens Neoplasm Animals Immunology and Allergy Gold Heat-Shock Proteins
Zdroj:	ResearcherID
ISSN:	1550-6606 0022-1767
Popis:	Immunization with heat shock proteins (HSPs) induces Ag-specific CTL responses. The specificity of the immune response is based on peptides associated with HSPs. To investigate how exogenous HSP/peptide complexes gain access to the MHC class I-restricted Ag presentation pathway, we incubated the monocytic cell line P388D1 and the dendritic cell line D2SC/1 with gold-labeled HSPs gp96 and HSC70. We show that HSPs bind specifically to the surface of these APCs and are internalized spontaneously by receptor-mediated endocytosis, demonstrating the existence of specific receptors for HSPs on these cells. In addition, we observe colocalization of internalized HSPs and surface MHC class I molecules in early and late endosomal structures. These findings provide possible explanations for the immunogenicity of HSP/peptide complexes and for the transfer of HSP-associated peptides onto MHC class I molecules.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec4443dfe33d255bf8cde7872c1c0142 https://doi.org/10.4049/jimmunol.162.7.3757 Zobrazit plný text záznamu