Knockdown of the Ribosomal Protein eL29 in Mammalian Cells Leads to Significant Changes in Gene Expression at the Transcription Level
| Autor: | Marsel R. Kabilov, Alexey A. Malygin, Galina G. Karpova, Alya G. Venyaminova, Alexey E. Tupikin, Maria I. Meschaninova, Alexander V. Gopanenko, Alena V. Kolobova |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Ribosomal Proteins
Transcription Genetic knockdown of ribosomal protein eL29 eL29-related processes Biology Article Transcriptome Proto-Oncogene Proteins c-myc Transcription (biology) Ribosomal protein HEK293 cells Gene expression Animals Humans differential gene expression Gene lcsh:QH301-705.5 Mammals Gene knockdown RNA RNA-Binding Proteins General Medicine Ribosomal RNA Cell biology Gene Ontology Gene Expression Regulation lcsh:Biology (General) Gene Knockdown Techniques next-generation sequencing eL29-dependent genes Tumor Suppressor Protein p53 RNA-seq |
| Zdroj: | Cells, Vol 9, Iss 1228, p 1228 (2020) Cells Volume 9 Issue 5 |
| ISSN: | 2073-4409 |
| Popis: | An imbalance in the synthesis of ribosomal proteins can lead to the disruption of various cellular processes. For mammalian cells, it has been shown that the level of the eukaryote-specific ribosomal protein eL29, also known as the one interacting with heparin/heparan sulfate, substantially affects their growth. Moreover, in animals lacking this protein, a number of anatomical abnormalities have been observed. Here, we applied next-generation RNA sequencing to HEK293 cells transfected with siRNAs specific for the mRNA of eL29 to determine what changes occur in the transcriptome profile with a decrease in the level of the target protein. We showed that an approximately 2.5-fold decrease in the content of eL29 leads to statistically significant changes in the expression of more than a thousand genes at the transcription level, without a noticeable effect on cell viability, rRNA level, and global translation. The set of eL29-dependent genes included both up-regulated and down-regulated ones, among which there are those previously identified as targets for proteins implicated in oncogenesis. Thus, our findings demonstrate that an insufficiency of eL29 in mammalian cells causes a significant reorganization of gene expression, thereby highlighting the relationship between the cellular balance of eL29 and the activities of certain genes. |
| Databáze: | OpenAIRE |
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