Shotgun metagenomics reveals both taxonomic and tryptophan pathway differences of gut microbiota in major depressive disorder patients
| Autor: | Tie-bang Liu, Wen-tao Lai, Min-zhi Li, Wen-feng Deng, Jie Zhao, Dan Xu, Fusheng He, Yang-hui Liu, Mingbang Wang, Yuan-yuan Guo, Han Rong, Xin-hui Xie, Ying-li Zhang, Qi-fan Yang, Shu-xian Xu, Sheng Wang, Shu-wei Ye, Ying-jia Yang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Gut flora behavioral disciplines and activities law.invention 03 medical and health sciences Probiotic 0302 clinical medicine law mental disorders Hamd medicine Humans KEGG Applied Psychology Bifidobacterium Aged Genetics Depressive Disorder Major biology Tryptophan Bacteroidetes Middle Aged medicine.disease biology.organism_classification Gastrointestinal Microbiome Psychiatry and Mental health 030104 developmental biology Metagenomics Major depressive disorder Female 030217 neurology & neurosurgery |
| Zdroj: | Psychological medicine. 51(1) |
| ISSN: | 1469-8978 0033-2917 |
| Popis: | BackgroundThe microbiota–gut–brain axis, especially the microbial tryptophan (Trp) biosynthesis and metabolism pathway (MiTBamp), may play a critical role in the pathogenesis of major depressive disorder (MDD). However, studies on the MiTBamp in MDD are lacking. The aim of the present study was to analyze the gut microbiota composition and the MiTBamp in MDD patients.MethodsWe performed shotgun metagenomic sequencing of stool samples from 26 MDD patients and 29 healthy controls (HCs). In addition to the microbiota community and the MiTBamp analyses, we also built a classification based on the Random Forests (RF) and Boruta algorithm to identify the gut microbiota as biomarkers for MDD.ResultsThe Bacteroidetes abundance was strongly reduced whereas that of Actinobacteria was significantly increased in the MDD patients compared with the abundance in the HCs. Most noteworthy, the MDD patients had increased levels of Bifidobacterium, which is commonly used as a probiotic. Four Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologies (KOs) (K01817, K11358, K01626, K01667) abundances in the MiTBamp were significantly lower in the MDD group. Furthermore, we found a negative correlation between the K01626 abundance and the HAMD scores in the MDD group. Finally, RF classification at the genus level can achieve an area under the receiver operating characteristic curve of 0.890.ConclusionsThe present findings enabled a better understanding of the changes in gut microbiota and the related Trp pathway in MDD. Alterations of the gut microbiota may have the potential as biomarkers for distinguishing MDD patients form HCs. |
| Databáze: | OpenAIRE |
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