The Ability of Chloroquine To Prevent Tat-Induced Cytokine Secretion by Monocytes Is Implicated in Its In Vivo Anti-Human Immunodeficiency Virus Type 1 Activity
| Autor: | Agnès Vendeville, Fabienne Rayne, Bruno Beaumelle, Anne Bonhoure |
|---|---|
| Přispěvatelé: | Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Anti-HIV Agents
medicine.medical_treatment [SDV]Life Sciences [q-bio] Immunology [SDV.BC]Life Sciences [q-bio]/Cellular Biology Pharmacology Biology Microbiology Peripheral blood mononuclear cell Monocytes Virus 03 medical and health sciences Chloroquine In vivo Virology Vaccines and Antiviral Agents medicine Humans Secretion ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Interleukin-6 030306 microbiology Monocyte 3. Good health medicine.anatomical_structure Cytokine Insect Science Gene Products tat [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology HIV-1 Cytokines tat Gene Products Human Immunodeficiency Virus Cytokine secretion medicine.drug |
| Zdroj: | Journal of Virology Journal of Virology, American Society for Microbiology, 2004, 78 (21), pp.12054-12057. ⟨10.1128/JVI.78.21.12054-12057.2004⟩ |
| ISSN: | 0022-538X 1098-5514 |
| Popis: | Hydroxychloroquine at 1 μM reduces the load of human immunodeficiency virus type 1 (HIV-1) in patients, whereas chloroquine (CQ) concentrations above 3 μM are required for inhibition of HIV-1 replication in peripheral blood mononuclear cells. Exogenous HIV-1 Tat reaches the cytosol of T cells by using low endosomal pH, and endosome neutralization by CQ prevents Tat from entering and affecting T cells. We show here that 0.6 μM CQ inhibits cytokine secretion induced by Tat in monocytes without affecting lipopolysaccharide-triggered cytokine release. This finding suggests that the in vivo anti-HIV-1 effect of CQ results not from a direct effect on the infected cell but rather from the capacity of CQ to prevent Tat from perturbing the cytokine balance. |
| Databáze: | OpenAIRE |
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