Measurement of interleukin-17
| Autor: | Chen Dong, Bhanu P. Pappu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
CD4-Positive T-Lymphocytes
Cellular differentiation medicine.medical_treatment Immunology Interleukin-17 Cell Culture Techniques Cell Differentiation Cell Separation Biology medicine.disease_cause Flow Cytometry Molecular biology In vitro Proinflammatory cytokine Autoimmunity Mice Cytokine Gene Expression Regulation Cell culture T-Lymphocyte Subsets T cell differentiation medicine Animals Interleukin 17 Inflammation Mediators |
| Zdroj: | Current Protocols in Immunology |
| ISSN: | 1934-368X |
| Popis: | Upon antigenic stimulation, naive CD4+ T cells undergo proliferation and differentiate into cytokine-producing T helper (T(H)) effector cells. T(H)1 cells secrete effector cytokine IFN-gamma and regulate cell-mediated immunity, whereas T(H)2 cells produce IL-4, IL-5, and IL-13 cytokines, and mediate immunity against extracellular pathogens and allergic reactions. Recent studies have identified a novel T(H) subset, called T(H)17, TH(IL-17), or inflammatory T(H) (THi) cells, characterized by the production of a proinflammatory cytokine, IL-17, and regulating inflammatory responses. In this unit, we describe the protocols for the differentiation of mouse IL-17-expressing T cells in vitro, detection of IL-17-expressing T cells by intracellular cytokine staining, and measurement of IL-17 secretion in culture supernatants by ELISA. Generation of IL-17-expressing T cells in vitro under defined culture conditions allows investigation of their differentiation regulation. Detection of IL-17 in cell culture and tissue samples helps in monitoring inflammatory diseases and determining efficacy of therapeutic interventions. |
| Databáze: | OpenAIRE |
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