DNA damage exerted by mixtures of commercial formulations of glyphosate and imazethapyr herbicides in Rhinella arenarum (Anura, Bufonidae) tadpoles
| Autor: | Marcelo L. Larramendy, Celeste Ruiz de Arcaute, Milagros Rosa Raquel Laborde, Wanessa F. Carvalho, Juan Manuel Pérez-Iglesias, Sonia Soloneski |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Veterinary medicine Health Toxicology and Mutagenesis Glycine 010501 environmental sciences Management Monitoring Policy and Law Toxicology 01 natural sciences Ciencias Biológicas Amphibians Lethal Dose 50 chemistry.chemical_compound Pivot® H Biología Celular Microbiología TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY Credit® Bioassay Ecotoxicology Animals Comet assay 0105 earth and related environmental sciences biology Herbicides Mutagenicity Tests Nicotinic Acids General Medicine Química Pesticide biology.organism_classification Acute toxicity Bufonidae 010602 entomology Rhinella arenarum chemistry Glyphosate Larva Toxicity CIENCIAS NATURALES Y EXACTAS Herbicide binary mixture Water Pollutants Chemical DNA Damage |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| ISSN: | 1573-3017 |
| Popis: | Glyphosate (GLY) and imazethapyr (IMZT) are two herbicides commonly used worldwide, either alone or in mixtures. They represent key pesticides in modern agricultural management. The toxicity that results when employed as mixtures has not been characterized so far. Acute toxicity of the 48% GLY-based herbicide (GBH) Credit® and the 10.59% IMZT-based herbicide (IBH) Pivot® H alone and their binary combinations was analyzed in Rhinella arenarum tadpoles exposed in a semi-static renewal test. Lethal effects were determined using mortality as the end-point, whereas sublethal effects were determined employing the single-cell gel electrophoresis (SCGE) bioassay. Based on mortality experiments, results revealed LC5096 h values of 78.18 mg/L GBH and 0.99 mg/L IBH for Credit® and Pivot® H, respectively. An increase in the genetic damage index (GDI) was found after exposure to Credit® or Pivot® H at 5 and 10% of LC5096 h values. The combinations of 5% Credit®-5% Pivot® H LC5096 h and 10% Credit®-10% Pivot® H LC5096 h concentrations significantly enhanced the GDI in comparison with tadpoles exposed only to Credit® or Pivot® H. Thus, the effect of interaction between GBH and IBH inducing DNA damage in R. arenarum blood cells can be considered to be synergistic. Facultad de Ciencias Naturales y Museo |
| Databáze: | OpenAIRE |
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