Hyperbilirubinemia-induced pro-angiogenic activity of infantile endothelial progenitor cells

Autor: Vahid Siavashi, Homa Babaei, Masoumeh Jabarpour, Seyed Mahdi Nassiri, Simin Asadian, Seyedeh Maedeh Jafari
Rok vydání: 2017
Předmět:
0301 basic medicine
MAPK/ERK pathway
Male
Vascular Endothelial Growth Factor A
Bilirubin
VEGF receptors
Neovascularization
Physiologic
Wounds
Penetrating
Biochemistry
Endothelial progenitor cell
03 medical and health sciences
chemistry.chemical_compound
Cell Movement
Medicine
Animals
Humans
Progenitor cell
Angiogenic Proteins
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Cells
Cultured
Cell Proliferation
Endothelial Progenitor Cells
Wound Healing
biology
business.industry
Cell growth
Tumor Necrosis Factor-alpha
Infant
Newborn
Cell Biology
Interleukin-10
Mice
Inbred C57BL
030104 developmental biology
chemistry
Case-Control Studies
Culture Media
Conditioned
Cancer research
biology.protein
Tumor necrosis factor alpha
Hyperbilirubinemia
Neonatal
Cardiology and Cardiovascular Medicine
business
Wound healing
Signal Transduction
Zdroj: Microvascular research. 118
ISSN: 1095-9319
Popis: Objectives Bilirubin, a by-product of heme degradation, is suggested to have a role for vascular protection. There is increasing evidence that bilirubin may directly affect the function and secretory activity of endothelial cells. In this study, potential effect of hyperbilirubinemia on biological features of circulation endothelial progenitor cells (cEPCs) isolated from infants was investigated. Methods Circulation concentration, differentiation and migratory activity of cEPCs isolated from infants with (n = 111) or without (n = 73) hyperbilirubinemia were analyzed. Then, the potential beneficial effect of conditioned medium of cEPCs from infants with or without hyperbilirubinemia was examined on experimental mouse wounds. Results Our results revealed significantly higher percentages of cEPCs in infants with hyperbilirubinemia. Cell proliferation, and migratory properties of cEPCs isolated and expanded from infants with hyperbilirubinemia were significantly improved. Also, the conditioned medium of cEPCs from hyperbilirubinemic infants possessed a superior beneficial effect on wound healing, which was associated with increased protein levels of VEGF, IL-10, and Pho-ERK/ERK, and decreased TNF-α in the wound tissues. Conclusions Our results showed that hyperbilirubinemia can activate migration, proliferating and angiogenic properties of cEPCs. Hyperbilirubinemia can promote the proangiogenic secretory activity of cEPCs, thereby resulting in enhancement of their regenerative wound healing properties.
Databáze: OpenAIRE
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