EPA prevents fat mass expansion and metabolic disturbances in mice fed with a Western diet[S]
| Autor: | Chrystèle Jouve, Elodie Pitois, Jean-Paul Rigaudière, Brigitte Laillet, Alexandre Pinel, Christophe Montaurier, Sarah De Saint-Vincent, Alain Huertas, Béatrice Morio, Frédéric Capel |
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| Přispěvatelé: | Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Lesieur |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Leptin
Male 0301 basic medicine obesity eicosapentaenoic acid Erythrocytes Drug Evaluation Preclinical Gene Expression Mice Obese Adipose tissue Biochemistry Mice Endocrinology insulin resistance Glucose homeostasis Phospholipids Research Articles Adiposity 2. Zero hunger Adipogenesis omega-3 fatty acids adipose tissue nutrition régulation du metabolisme [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Eicosapentaenoic acid 3. Good health Alimentation et Nutrition Endocrinologie et métabolisme lipids (amino acids peptides and proteins) medicine.symptom medicine.medical_specialty Adipose Tissue White omega-3 polyunsaturated fatty acid Adipokine QD415-436 Biology 03 medical and health sciences Insulin resistance Adipokines 3T3-L1 Cells Internal medicine Glucose Intolerance medicine Animals Food and Nutrition Endocrinology and metabolism 030109 nutrition & dietetics Adiponectin Cell Membrane Cell Biology Lipid Metabolism medicine.disease Mice Inbred C57BL Diet Western acide gras polyinsaturé n-3 Anti-Obesity Agents Weight gain [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Journal of Lipid Research, Vol 57, Iss 8, Pp 1382-1397 (2016) Journal of Lipid Research 8 (57), 1382-1397. (2016) Journal of Lipid Research Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 2016, 57 (8), pp.1382-1397. ⟨10.1194/jlr.M065458⟩ Journal of Lipid Research, 2016, 57 (8), pp.1382-1397. ⟨10.1194/jlr.M065458⟩ |
| ISSN: | 0022-2275 |
| DOI: | 10.1194/jlr.M065458⟩ |
| Popis: | The authors acknowledge the staff from Auvergne University Experimental Animal Laboratory and Christophe Del’Homme, Philippe Denis, Anne Terisse-Lottier and Alexandre Teynie from the Experimental Animal Facility of the Human Nutrition Unit (INRA of Clermont-Ferrand) for their assistance throughout the animal protocol. We thank Celine Bobby for her help for TaqMan gene expression assays. We gratefully acknowledge financial support and doctoral fellowship (to A.P.) fromLesieur and Avril; Impact of ALA, EPA and DHA on obesity and metabolic complications were studied in mice fed a high-fat high-sucrose diet (HF). HF diets were supplemented with ALA, EPA or DHA (1%w/w) and given to C57BL/6J mice for 16 weeks and to Ob/Ob mice for 6 weeks. In C57BL/6J mice, EPA reduced plasma cholesterol (-20%), limited fat mass accumulation (-23%), adipose cell hypertrophy (-50%), and reduced plasma leptin concentration (-60%), compared to HF fed mice. Furthermore, mice supplemented with EPA exhibited a higher insulin sensitivity (+24%) and glucose tolerance (+20%) compared to HF fed mice. Similar effects were observed in EPA supplemented Ob/Ob mice, although fat mass accumulation was not prevented. By contrast in comparison to HF fed mice, DHA did not prevent fat mass accumulation, increased plasma leptin concentration (+128%) in C57BL/6J mice and did not improve glucose homeostasis in C57BL/6J and Ob/Ob mice. In 3T3-L1 adipocytes, DHA stimulated leptin expression whereas EPA induced adiponectin expression, suggesting that improved leptin / adiponectin balance may contribute to the protective effect of EPA. In conclusion, supplementation with EPA, but not ALA and DHA, could preserve glucose homeostasis in an obesogenic environment and limit fat mass accumulation in the early stage of weight gain. |
| Databáze: | OpenAIRE |
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