An enzyme–detergent method for effective prion decontamination of surgical steel
| Autor: | Petra Hirsch, Edward McKintosh, Eckhard Flechsig, Charles Weissmann, Kanella Prodromidou, Jackie Linehan, Anthony R. Clarke, John Collinge, Graham S. Jackson, Sebastian Brandner |
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| Rok vydání: | 2005 |
| Předmět: |
Prions
animal diseases Bovine spongiform encephalopathy Detergents Population Pronase Biology Creutzfeldt-Jakob Syndrome Virology mental disorders medicine Bioassay education Decontamination Infectivity education.field_of_study Proteolytic enzymes Sterilization (microbiology) Surgical Instruments Proteinase K medicine.disease Organophosphates nervous system diseases biology.protein Endopeptidase K |
| Zdroj: | Journal of General Virology. 86:869-878 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.80484-0 |
| Popis: | Prions, transmissible agents that cause Creutzfeldt-Jakob disease (CJD) and other prion diseases, are known to resist conventional sterilization procedures. Iatrogenic transmission of classical CJD via neurosurgical instruments is well documented and the involvement of lymphoreticular tissues in variant CJD (vCJD), together with the unknown population prevalence of asymptomatic vCJD infection, has led to concerns about transmission from a wide range of surgical procedures. To address this problem, conditions were sought that destroy PrP(Sc) from vCJD-infected human tissue and eradicate RML prion infectivity adsorbed onto surgical steel. Seven proteolytic enzymes were evaluated individually and in pairs at a range of temperatures and pH values and the additional effects of detergents, lipases and metal ions were assessed. A combination of proteinase K and Pronase, in conjunction with SDS, was shown to degrade PrP(Sc) material from highly concentrated vCJD-infected brain preparations to a level below detection. When RML prion-infected wires were exposed to the same enzymic treatment, intracerebral bioassay in highly susceptible hosts showed virtually no infectivity. The prion-degrading reagents identified in this study are readily available, inexpensive, non-corrosive to instruments, non-hazardous to staff and compatible with current equipment and procedures used in hospital sterilization units. |
| Databáze: | OpenAIRE |
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