Human dendritic cell maturation and activation by a heat-killed recombinant yeast (Saccharomyces cerevisiae) vector encoding carcinoembryonic antigen
| Autor: | Helen Sabzevari, Cinzia Remondo, Alex Franzusoff, Kwong-Y. Tsang, Jeffrey Schlom, Sven Mostböck, Vittore Cereda |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_treatment
CD58 Antigen presentation chemical and pharmacologic phenomena Saccharomyces cerevisiae Lymphocyte Activation Article Carcinoembryonic antigen Cancer immunotherapy Antigen Antigens CD Interferon Cell Line Tumor medicine Humans neoplasms MHC class II General Veterinary General Immunology and Microbiology biology Gene Expression Profiling Public Health Environmental and Occupational Health hemic and immune systems Dendritic Cells Dendritic cell Cytotoxicity Tests Immunologic digestive system diseases Carcinoembryonic Antigen Cell biology Infectious Diseases Cancer research biology.protein Cytokines Molecular Medicine T-Lymphocytes Cytotoxic medicine.drug |
| Zdroj: | Vaccine. 27:987-994 |
| ISSN: | 0264-410X |
| Popis: | Tumor-associated antigens are weakly immunogenic. Human carcinoembryonic antigen (CEA) is overexpressed on a wide range of human carcinomas and represents an attractive target for cancer immunotherapy. This study analyzes the ability of a Saccharomyces cerevisiae vector containing the transgene encoding CEA (yeast-CEA) to activate human dendritic cells (DCs) and stimulate CEA-specific T-cell responses. We demonstrate for the first time that treatment with yeast-CEA can activate human DCs, resulting in increases in surface expression of CD80, CD83, CD54, CD58, and MHC class II, and increased production by DCs of IL-12p70, TNF-alpha, IFN-gamma, IL-8, IL-2, IL-13, IL-10, and IL-1beta. We also show that human DCs treated with yeast-CEA can activate CEA-specific T-cell lines and can act as antigen-presenting cells (APCs) to generate CEA-specific T-cell lines capable of lysing CEA(+) human tumor cells. Gene expression profiles of human DCs treated with yeast-CEA show increased expression of numerous genes involved in the production of chemokines and cytokines and their receptors, and genes related to antigen uptake, antigen presentation, and signal transduction. |
| Databáze: | OpenAIRE |
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