Differential Expression of the Chemokine Receptors CX3CR1 and CCR1 by Microglia and Macrophages in Myelin-Oligodendrocyte-Glycoprotein-Induced Experimental Autoimmune Encephalomyelitis
| Autor: | Lena Appelsved, Erik Wallström, Fredrik Piehl, Dan Sunnemark, Anders Ericsson-Dahlstrand, Hans Lassmann, Åsa Malmberg, Sana Eltayeb, Tomas Olsson |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
CCR1
CCR2 Time Factors Receptor expression Chemokine receptor CX3CR1 Image Processing Computer-Assisted Hepatocyte Nuclear Factor 1-alpha Lymphocytes In Situ Hybridization Microglia biology Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Experimental autoimmune encephalomyelitis Nuclear Proteins Ectodysplasins Flow Cytometry Immunohistochemistry Cell biology DNA-Binding Proteins Myelin-Associated Glycoprotein medicine.anatomical_structure Hepatocyte Nuclear Factor 1 Female Receptors Chemokine Myelin Proteins Research Article Encephalomyelitis Autoimmune Experimental CX3C Chemokine Receptor 1 Receptors CCR1 Pathology and Forensic Medicine Myelin oligodendrocyte glycoprotein Receptors HIV medicine Animals RNA Messenger Receptors Cytokine Hepatocyte Nuclear Factor 1-beta Staining and Labeling Macrophages Membrane Proteins Rats Inbred Strains medicine.disease Rats Disease Models Animal Immunology biology.protein Immunization Myelin-Oligodendrocyte Glycoprotein Neurology (clinical) Transcription Factors |
| Zdroj: | Karolinska Institutet Europe PubMed Central Brain Pathol |
| ISSN: | 1015-6305 |
| DOI: | 10.1111/j.1750-3639.2003.tb00490.x |
| Popis: | Chemokines are important for the recruitment of immune cells into sites of inflammation. To better understand their functional roles during inflammation we have here studied the in vivo expression of receptors for the chemokines CCL3/CCL5/CCL7 (MIP‐1α/RANTES/MCP‐3) and CX(3)CL1 (fractalkine), CCR1 and CX(3)CR1, respectively, in rat myelin oligodendrocyte glycoprotein‐induced experimental autoimmune encephalomyelitis. Combined in situ hybridization and immunohistochemistry demonstrated intensely upregulated CCR1 mRN A expression in early, actively demyelinating plaques, whereas CX(3)CR1 displayed a more generalized expression pattern. CX(3)CR1 mRNA expressing cells were identified as microglia on the basis of their cellular morphology and positive GSA/B4 lectin staining. In contrast, CCR1 mRNA was preferentially expressed by ED1(+)GSA/B4(+) macrophages. The notion of differential chemokine receptor expression in microglia and monocyte‐derived macrophages was corroborated at the protein level by extraction and flow cytometric sorting of cells infiltrating the spinal cord using gating for the surface markers CD45, ED‐2 and CD11b. These observations suggest a differential receptor expression between microglia and monocyte‐derived macrophages and that mainly the latter cell type is responsible for active demyelination. This has great relevance for the possibility of therapeutic intervention in demyelinating diseases such as multiple sclerosis, for example by targeting signaling events leading to monocyte recruitment. |
| Databáze: | OpenAIRE |
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