Immunohistochemical Localization of Glutathione S-Transferase α and π in Human Esophageal Squamous Epithelium, Barrett's Epithelium and Carcinoma

Autor: Th. Wobbes, W.H.M. Peters, U. J. G. M. Van Haelst, E.M.M. van Lieshout
Rok vydání: 1999
Předmět:
Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Esophageal Neoplasms
Esophageal cancer
mogelijke oorzaken en gevolgen (sepsis en ontsteking) [Sepsis en niet-bacteriële gegeneraliseerde ontsteking]
Adenocarcinoma
causes and effects (sepsis and inflammation) [Sepsis and non-bacterial generalized inflammation]
Article
Epithelium
Nutrition
secretion and motility
Barrett's esophagus
Barrett Esophagus
Voeding
secretie en motoriek
medicine
Humans
Glutathione S‐transferases
Tumor pathology
Esophagus
Aged
Glutathione Transferase
biology
Chemistry
Middle Aged
Chirurgische Oncologie
Tumor pathologie
medicine.disease
Immunohistochemistry
digestive system diseases
Isoenzymes
Surgical Oncology
medicine.anatomical_structure
Glutathione S-transferase
Glutathione S-Transferase pi
Oncology
Carcinoma
Squamous Cell
biology.protein
Female
Precancerous Conditions
Immunostaining
Zdroj: Japanese Journal of Cancer Research, 90, pp. 530-535
Japanese Journal of Cancer Research : Gann
Japanese Journal of Cancer Research, 90, 530-535
ISSN: 0910-5050
DOI: 10.1111/j.1349-7006.1999.tb00780.x
Popis: High tissue levels of glutathione S-transferases (GSTs), a family of detoxification enzymes, are inversely correlated with cancer risk in the human gastrointestinal tract. Patients with Barrett's esophagus, wherein squamous epithelium is replaced by columnar epithelium, have an increased risk for developing esophageal adenocarcinoma. Biochemical analyses revealed that Barrett's epithelium contains lower levels of GST enzyme activity as well as some GST isoforms, as compared with squamous epithelium. So far, little information on the immunohistochemical distribution of the GST alpha and pi isoforms in normal squamous epithelium, in Barrett's metaplastic epithelium or in adeno- and squamous cell carcinomas of the esophagus is available. Tissues were fixed in formalin and embedded in paraffin. Three 4 microm thick sections were used for hematoxylin and eosin staining and for immunostaining with antibodies against GST alpha and pi. GST alpha and pi were seen in normal squamous epithelium (0% and 75%, respectively), Barrett's epithelium (75% and 100%), adenocarcinoma (25% and 100) and squamous cell carcinoma (27% and 91%). Staining was mainly cytoplasmic, though some nuclear staining with the GST pi antibody was apparent. The varying expression of GST alpha and pi in normal and (pre)neoplastic esophagus may have consequences for the treatment of these diseases and may contribute to an understanding of the development of these esophageal disorders.
Databáze: OpenAIRE
Externí odkaz: