FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk
| Autor: | Raman M. Das, Kate G. Storey, Aixa V. Morales, Julio A. Barbas, Ruth Diez del Corral, Alejandra C. Quiroga, Isabel Olivera-Martinez, Patricia L. Martínez-Morales |
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| Rok vydání: | 2011 |
| Předmět: |
Central Nervous System
medicine.medical_specialty Mesoderm Epithelial-Mesenchymal Transition MAP Kinase Signaling System Tretinoin Chick Embryo Biology Fibroblast growth factor Polymerase Chain Reaction Article FGF and mesoderm formation 03 medical and health sciences 0302 clinical medicine Cell Movement Internal medicine Peripheral Nervous System medicine Paraxial mesoderm Animals Epithelial–mesenchymal transition Research Articles 030304 developmental biology 0303 health sciences Cell Cycle Neural tube Wnt signaling pathway Gene Expression Regulation Developmental Neural crest Cell Biology Cell biology Fibroblast Growth Factors Wnt Proteins Electroporation Endocrinology medicine.anatomical_structure Neural Crest Bone Morphogenetic Proteins embryonic structures Mitogen-Activated Protein Kinases 030217 neurology & neurosurgery Signal Transduction Transcription Factors |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname The Journal of Cell Biology |
| Popis: | Coordination between functionally related adjacent tissues is essential during development. For example, formation of trunk neural crest cells (NCCs) is highly influenced by the adjacent mesoderm, but the molecular mechanism involved is not well understood. As part of this mechanism, fibroblast growth factor (FGF) and retinoic acid (RA) mesodermal gradients control the onset of neurogenesis in the extending neural tube. In this paper, using gain- and loss-of-function experiments, we show that caudal FGF signaling prevents premature specification of NCCs and, consequently, premature epithelial¿mesenchymal transition (EMT) to allow cell emigration. In contrast, rostrally generated RA promotes EMT of NCCs at somitic levels. Furthermore, we show that FGF and RA signaling control EMT in part through the modulation of elements of the bone morphogenetic protein and Wnt signaling pathways. These data establish a clear role for opposition of FGF and RA signaling in control of the timing of NCC EMT and emigration and, consequently, coordination of the development of the central and peripheral nervous system during vertebrate trunk elongation. This work was funded by the Spanish MCINN grants to A.V.M. (BFU2005- 00762 and BFU2008-02963) and to R.D.C. (BFU2005-02972). A.V.M. was supported by the RyC Programme, P.L.M by a FPU fellowship, K.G.S and I.O-M were funded by MRC grant G0600234, R.A.D by Wellcome Trust grant to KGS 083611/Z/07/Z. RyC Programme FPU fellowship Wellcome Trust Spanish MCINN |
| Databáze: | OpenAIRE |
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