Validation of a binary ethylenimine (BEI) inactivation procedure for biosafety treatment of foot-and-mouth disease viruses (FMDV), vesicular stomatitis viruses (VSV), and swine vesicular disease virus (SVDV)
| Autor: | Benjamin J. Hershey, Wei Jia, Ping Wu, Yelitza Y. Rodríguez, Yadata Tadassa, Kimberly A. Dodd |
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| Rok vydání: | 2021 |
| Předmět: |
Swine
viruses Aziridines Microbiology Vesicular stomatitis Indiana virus Virus 03 medical and health sciences Vesicular Stomatitis Virucide Animals Binary ethylenimine 030304 developmental biology Swine Diseases 0303 health sciences General Veterinary biology 030306 microbiology General Medicine Containment of Biohazards biology.organism_classification Virology Enterovirus B Human Titer Swine Vesicular Disease Virus Foot-and-Mouth Disease Virus Vesicular stomatitis virus Foot-and-Mouth Disease Virus Inactivation Foot-and-mouth disease virus |
| Zdroj: | Veterinary Microbiology. 252:108928 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2020.108928 |
| Popis: | Binary ethylenimine (BEI) has been widely used as a virucide to inactivate viruses. For regulatory exclusion of a select agent, the United States Federal Select Agent Program (FSAP) requires an inactivation procedure that renders a select agent non-viable but allows the select agent to retain antigenic characteristics for future use must be validated, and the inactivated agent must be confirmed by a viability testing. In this curve-based validation study, we examined impacts of BEI concentration, treatment temperature, and time on our in-house inactivation procedures of Foot-and-Mouth Disease Virus (FMDV), Vesicular Stomatitis Virus (VSV), and Swine Vesicular Disease Virus (SVDV). The inactivation efficacy was confirmed by virus titration and 3 consecutive blind passages on the monolayers of susceptible cells. A linear correlation between the virus titer reduction and BEI concentration, treatment time, and temperature was established. The results confirmed our in-house BEI inactivation procedure of two doses of 1.5 mM BEI treatment at 37 °C, 1st dose for 24 h, then 2nd dose for 6 more hours for a total of 30 h BEI contact time, can ensure complete inactivation of FMDV, VSV, and SVDV. |
| Databáze: | OpenAIRE |
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