Transposable Element Mobilization in Interspecific Yeast Hybrids
| Autor: | Maitreya J. Dunham, Angela Shang-Mei Hickey, Erica Alcantara, Kira Patterson, Caiti Smukowski Heil |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI01140
Transposable element Mitochondrial DNA Retroelements Saccharomyces cerevisiae Retrotransposon Saccharomyces Genome Transposition (music) 03 medical and health sciences 0302 clinical medicine Species Specificity Genetics hybridization Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Whole Genome Sequencing biology transposition rate AcademicSubjects/SCI01130 Terminal Repeat Sequences food and beverages Genomics biology.organism_classification Long terminal repeat DNA Transposable Elements Hybridization Genetic transposable elements Sequence Analysis 030217 neurology & neurosurgery Research Article Ty element |
| Zdroj: | Genome Biology and Evolution |
| ISSN: | 1759-6653 |
| DOI: | 10.1093/gbe/evab033 |
| Popis: | Barbara McClintock first hypothesized that interspecific hybridization could provide a “genomic shock” that leads to the mobilization of transposable elements (TEs). This hypothesis is based on the idea that regulation of TE movement is potentially disrupted in hybrids. However, the handful of studies testing this hypothesis have yielded mixed results. Here, we set out to identify if hybridization can increase transposition rate and facilitate colonization of TEs in Saccharomyces cerevisiae × Saccharomyces uvarum interspecific yeast hybrids. Saccharomyces cerevisiae have a small number of active long terminal repeat retrotransposons (Ty elements), whereas their distant relative S. uvarum have lost the Ty elements active in S. cerevisiae. Although the regulation system of Ty elements is known in S. cerevisiae, it is unclear how Ty elements are regulated in other Saccharomyces species, and what mechanisms contributed to the loss of most classes of Ty elements in S. uvarum. Therefore, we first assessed whether TEs could insert in the S. uvarum sub-genome of a S. cerevisiae × S. uvarum hybrid. We induced transposition to occur in these hybrids and developed a sequencing technique to show that Ty elements insert readily and nonrandomly in the S. uvarum genome. We then used an in vivo reporter construct to directly measure transposition rate in hybrids, demonstrating that hybridization itself does not alter rate of mobilization. However, we surprisingly show that species-specific mitochondrial inheritance can change transposition rate by an order of magnitude. Overall, our results provide evidence that hybridization can potentially facilitate the introduction of TEs across species boundaries and alter transposition via mitochondrial transmission, but that this does not lead to unrestrained proliferation of TEs suggested by the genomic shock theory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|