| Autor: |
Andrade da Silva, Luisa H., Heuer, Rachel A., Roque, Christian B., McGuire, Tammy L., Hosoya, Tomonori, Kimura, Hironobu, Tamura, Kouichi, Matsuoka, Akihiro J. |
| Rok vydání: |
2023 |
| DOI: |
10.6084/m9.figshare.22623832.v1 |
| Popis: |
Additional file 1. Supplemental information. Section 1: Supplementary methods. 1.1. Differentiation of hi-iPSCs toward otic neural progenitors. 1.2. Quantitative RT-qPCR. 1.3. Immunocytochemistry. 1.4. Flow cytometry analysis. 1.5. Generation of ONP spheroids for transplantation. 1.6. Intracochlear transplantation of ONPs through round window. 1.7. Immunohistochemistry and donor cell quantification; Section 2: Supplementary figures. Figure S1. Overexpression of HLA-G and PD-L1 genes in hypoimmunogenic iPSCs. Figure S2. G-banded karyotyping of hypoimmunogenic iPSCs. Figure S3. Surface expression of HLA-G and PD-L1 transgenes diminishes in hypoimmunogenic cells during differentiation from iPSC to ONP stage. Figure S4. Intratympanic transplantation of ONP spheroids. Figure S5. Representation of the mid-modiolar cryosections used for quantification of donor cells. Figure S6. Intracochlear transplantation of wild-type iPSC–derived ONP spheroids (wt-ONP). Figure S7. Intracochlear transplantation of hypoimmunogenic iPSC–derived ONP spheroids (hi-ONP). Figure S8. Distribution of ONP count/mm² values. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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