Probiotic Bifidobacterium strains and galactooligosaccharides improve intestinal barrier function in obese adults but show no synergism when used together as synbiotics
| Autor: | Krista Shawron, Janina A. Krumbeck, Ali Keshavarzian, Jens Walter, Heather E. Rasmussen, Jennifer Clarke, Robert W. Hutkins |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Sucrose Synbiotics medicine.medical_treatment Autochthonous Oligosaccharides Prebiotic Allochthonous Gut flora Probiotic law.invention chemistry.chemical_compound Lactulose 0302 clinical medicine law RNA Ribosomal 16S Gut barrier function Food science Bifidobacterium biology Galactooligosaccharide Middle Aged Intestines lcsh:QR100-130 Female 030211 gastroenterology & hepatology medicine.drug Adult Microbiology (medical) Microbiology lcsh:Microbial ecology Tight Junctions Young Adult 03 medical and health sciences Bifidobacteria Double-Blind Method medicine Humans Obesity Intestinal permeability Research Probiotics biology.organism_classification medicine.disease Endotoxemia Gastrointestinal Microbiome Synbiotic Prebiotics 030104 developmental biology chemistry |
| Zdroj: | Microbiome, Vol 6, Iss 1, Pp 1-16 (2018) Microbiome |
| ISSN: | 2049-2618 |
| Popis: | Background One way to improve both the ecological performance and functionality of probiotic bacteria is by combining them with a prebiotic in the form of a synbiotic. However, the degree to which such synbiotic formulations improve probiotic strain functionality in humans has not been tested systematically. Our goal was to use a randomized, double-blind, placebo-controlled, parallel-arm clinical trial in obese humans to compare the ecological and physiological impact of the prebiotic galactooligosaccharides (GOS) and the probiotic strains Bifidobacterium adolescentis IVS-1 (autochthonous and selected via in vivo selection) and Bifidobacterium lactis BB-12 (commercial probiotic allochthonous to the human gut) when used on their own or as synbiotic combinations. After 3 weeks of consumption, strain-specific quantitative real-time PCR and 16S rRNA gene sequencing were performed on fecal samples to assess changes in the microbiota. Intestinal permeability was determined by measuring sugar recovery in urine by GC after consumption of a sugar mixture. Serum-based endotoxin exposure was also assessed. Results IVS-1 reached significantly higher cell numbers in fecal samples than BB-12 (P |
| Databáze: | OpenAIRE |
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