Preliminary Report: The Relevance of Tumor Necrosis Factor-α in Acquired Primary Lymphedema—A Histopathological Investigation
| Autor: | Kouichi Tamura, Hiromichi Wakui, Saori Asano, Ryusuke Yoshimi, Jiro Maegawa, Taro Mikami, Shinobu Matsubara |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Inflammation
Pathology medicine.medical_specialty Tumor Necrosis Factor-alpha Secondary lymphedema business.industry Anastomosis Surgical H&E stain medicine.disease Proinflammatory cytokine Lymphedema Lymphatic system medicine Cytokines Humans Primary lymphedema Lymph medicine.symptom Cardiology and Cardiovascular Medicine business Lymphatic Vessels |
| Zdroj: | Lymphatic Research and Biology. 18:232-238 |
| ISSN: | 1557-8585 1539-6851 |
| DOI: | 10.1089/lrb.2019.0046 |
| Popis: | Background: Lymphedema includes primary lymphedema (P-LE) and secondary lymphedema (S-LE), which is a chronic progressive disease. The former group is further classified as congenital and acquired P-LE (AP-LE); its etiology is unclear, and only a few studies on its pathophysiology exist. We hypothesized that an autoimmune disease or self-inflammatory mechanism occurs in lymphatic vessels, leading to obstruction. Methods and Results: We enrolled 46 patients with lymphedema who underwent lymphaticovenous anastomosis (LVA) from January to October 2015. Collecting lymph ducts were obtained during LVA. We performed hematoxylin/eosin staining and immunostaining for LYVE-1, IL-1β, IL-6, and TNF-α. There were no substantial histological differences between the two types of lymphedema, whereas some differences in expression of inflammatory cytokines, including interleukin (IL)-1β and tumor necrosis factor (TNF)-α, were observed. Only a few inflammatory cells could be seen around the vessels. Although no significant differences in expression of IL-1β were found between AP-LE and S-LE, TNF-α was more highly expressed in the smooth muscle layer in AP-LE patients than in S-LE patients. There were no significant morphological differences in the collecting ducts of lymphatic vessels between S-LE and P-LE. Nevertheless, higher levels of TNF-α accumulation were found in the thick smooth muscle layer of P-LE patients than in that of S-LE patients. Conclusion: TNF-α-related inflammation in collecting ducts of lymphatic vessels is an important characteristic of the pathology of P-LE. TNF-α inhibitors might improve symptoms of AP-LE. |
| Databáze: | OpenAIRE |
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