Intracellular trafficking of Shiga-toxin-B-subunit-functionalized spherulites

Autor: Brigitte Delord, Cécile Poirier, Ludger Johannes, Anthony Bouter, Damien van Effenterre, Estelle Dransart
Přispěvatelé: Centre de recherches Paul Pascal (CRPP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Trafic, Institut Curie, Institut Curie [Paris], Compartimentation et dynamique cellulaires (CDC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2008
Předmět:
Zdroj: Biology of the Cell
Biology of the Cell, Wiley, 2008, 100 (12), pp. 717-725. ⟨10.1042/BC20080009⟩
Biology of the Cell, 2008, 100 (12), pp. 717-725. ⟨10.1042/BC20080009⟩
ISSN: 1768-322X
0248-4900
DOI: 10.1042/BC20080009⟩
Popis: Background information. Spherulites are multi-lamellar lipidic vesicles that can encapsulate biomolecules and may be used as carriers for drug delivery. STxB (Shiga toxin B-subunit) is known to bind the glycosphingolipid Gb3 (globotriaosyl ceramide), which is overexpressed by various human tumours. After Gb3 binding, the toxin enters the cytoplasm via the retrograde route, bypassing the degrading environment of the late endosomes/lysosomes. STxB is non-toxic and has been identified as a promising tool for drug delivery. So far, applications have relied on direct coupling with therapeutic agents. In the present study, we have investigated the functionalization of spherulites by STxB and the intracellular trafficking of these structures. Results. We demonstrate that STxB-spherulites (ST×B-functionalized spherulites) are internalized into HeLa cells in a receptor-dependent manner. The intracellular distribution was studied by confocal microscopy for lipids, ligand and content. We observed an early separation between spherulites and STxB, leading to a late endosomal/lysosomal localization of lipids and content, whereas STxB remained partially at the plasma membrane. Conclusions. Although recognition of Gb3 is the cause of their specific adhesion to cell membranes, STxB-spherulites do not follow the retrograde transport route. Our results strongly suggest that STxB-spherulites are, at least in part, disrupted at the plasma membrane, leading to lipid and content targeting to the classical endocytic pathway. We discuss how these findings influence the development of innovative delivery strategies.
Databáze: OpenAIRE
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